Characterization of two avian reoviruses that exhibit strain-specific quantitative differences in their syncytium-inducing and pathogenic capabilities
Characterization of two avian reoviruses that exhibit strain-specific quantitative differences in their syncytium-inducing and pathogenic capabilities
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DOI：
10.1006/viro.1998.9371
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发表时间：
1998-10-25
期刊：
影响因子：
3.7
通讯作者：
Sullivan, K
中科院分区：
文献类型：
--
作者：
Duncan, R;Sullivan, K
我们先前提出，在所有已报道的禽呼肠孤病毒(ARV)分离株中，非必需的合胞体诱导表型的保守可能反映了一种增强病毒在体内传播的机制，这反过来可能有助于ARV的自然致病性。由于缺乏在融合诱导能力上有明确差异的可用病毒株，这一假说的直接测试受到了阻碍。我们现在报告两个ARV株ARV-176和ARV-138的特性，它们在融合特性上表现出不同的株系差异，这与它们在胚胎中的致病潜力有关。此外，两株病毒在细胞培养中具有相似的复制能力，表明弱融合基因ARV-138病毒的合胞体诱导能力被特异性抑制。为了测试这些病毒在重组研究中的使用，目的是评估细胞融合在病毒致病中的作用，我们使用了一个包含9个来自亲本ARV-138病毒的基因组片段和来自高融合和致病性的ARV-176亲本病毒的S1基因组片段的单一配合体进行了初步的遗传分析。该单组合具有ARV-176亲本病毒的全部融合潜力，并显示出增强的胚胎致病性，首次提供了ARV S1基因组片段参与合胞体形成和病毒致病的遗传证据。(C)1998年学术出版社
We previously proposed that the conservation of the nonessential syncytium-inducing phenotype among all reported avian reovirus (ARV) isolates may reflect a mechanism for enhanced virus dissemination in vivo, which in turn could contribute to the natural pathogenicity of ARV. Direct testing of this hypothesis has been hampered by the lack of available virus strains with defined differences in their fusion-inducing capability. We now report on the characterization of two ARV strains, ARV-176 and ARV-138, that exhibited strain-specific differences in their fusogenic properties, which correlated with their pathogenic potential in embryonated eggs. Moreover, both virus strains possessed similar replicative abilities in cell culture, suggesting that the weakly fusogenic ARV-138 virus is specifically inhibited in its syncytium-inducing ability. To test the use of these viruses for reassortant studies aimed at assessing the role of cell fusion in viral pathogenesis, a preliminary genetic analysis was undertaken using a monoreassortant that contained nine genome segments from the parental ARV-138 virus and the S1 genome segment from the highly fusogenic and pathogenic ARV-176 parental virus. The monoreassortant possessed the full fusogenic potential of the ARV-176 parental virus and displayed enhanced embryo pathogenicity, providing the first genetic evidence implicating the ARV S1 genome segment in both syncytium formation and viral pathogenesis. (C) 1998 Academic Press