Study of bacterial communities in Antarctic coastal waters by a combination of 16S rRNA and 16S rDNA sequencing
Study of bacterial communities in Antarctic coastal waters by a combination of 16S rRNA and 16S rDNA sequencing
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DOI：
10.1111/j.1462-2920.2006.01097.x
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发表时间：
2006-12-01
影响因子：
5.1
通讯作者：
Yakimov, Michail M.
中科院分区：
文献类型：
--
作者：
Gentile, Gabriella;Giuliano, Laura;Yakimov, Michail M.
通过对两个不同海域提取的RNA和DNA克隆文库进行16 -based系统发育分析，并相互比较，确定了南极细菌群落分布的生态学研究。在罗斯海的四个监测站采集浅层海水样本，其中三个位于罗德湾，一个位于埃文斯湾；每个位点制备了两个克隆文库（16S rDNA和16S rRNA），每个位点的DNA文库和RNA文库存在明显差异。所有种型中有93.6%存在于RNA文库中；相比之下，从总微生物群落（DNA文库）中仅检索到31种（70.5%）。在分析的地点检测到与南极海冰细菌群落典型的γ -变形菌门和拟杆菌门类群相关的DNA和RNA序列。来自两个不同区域的16S rDNA和rRNA文库被来自质体和线粒体起源的浮游植物16S序列富集，反映了藻华发生在采样期间（2003年南极夏季）。在罗德湾文库中发现的高百分比的DNA克隆显然与活性污泥和井水中典型的β变形菌有关，这可以用该地点存在污水净化系统来解释。所获得的结果清楚地表明，16S rDNA和16S rRNA基因测序的组合是对微生物群落组成有更可靠的认识的首选方法。
An ecological study on distribution of Antarctic bacterial communities was determined by 16S-based phylogenetic analyses of clone libraries derived from RNA and DNA extracted from two different marine areas and compared between each other. Superficial seawater samples were collected from four stations in Ross Sea, three of them located in Rod Bay and one in Evans Cove; for each station two clone libraries (16S rDNA and 16S rRNA) were prepared and evident divergences between DNA and RNA libraries of each site were obtained. Of all phylotypes 93.6% were found in RNA libraries; in contrast, only 31 phylotypes (70.5%) were retrieved from total microbial community (DNA libraries). DNA and RNA sequences related to gamma-Proteobacteria and Bacteroidetes groups, typical for Antarctic sea-ice bacterial communities, were detected in analysed sites. 16S rDNA and rRNA libraries derived from the two different areas were enriched by picophytoplanktonic 16S sequences of plastid and mitochondrion origins, reflecting that the algal blooms occurred during sampling (Antarctic summer 2003). The finding in Rod Bay libraries of high percentage of DNA clones apparently affiliated with beta-Proteobacteria typical for activated sludges and well water could be explained by the presence of a sewage depuration system at this site. Obtained results clearly demonstrate that combination of 16S rDNA and 16S rRNA gene sequencing is preferred approach to have a more reliable vision on the composition of microbial communities.