Physiological Characteristics, Phytase Activity, and Mineral Bioavailability of a Low-Phytate Soybean Line during Germination
Physiological Characteristics, Phytase Activity, and Mineral Bioavailability of a Low-Phytate Soybean Line during Germination
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DOI：
10.1007/s11130-020-00827-x
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发表时间：
2020-05-21
影响因子：
4
通讯作者：
Saneoka, Hirofumi
中科院分区：
文献类型：
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作者：
Dong, Qin;Saneoka, Hirofumi
植酸盐是磷在种子中的一种储存形式，当其降解时，对种子萌发起着至关重要的作用。低植酸含量的谷物种子抑制发芽和幼苗生长。在这里，低植酸（LP）和正常植酸（NP）大豆品系生长在蛭石中，以确定发芽是否受到低植酸含量的影响。评价了NP和LP大豆品系幼苗的生长、植酸酶活性、矿物质浓度和矿物质可提取性。幼苗生长没有显着差异NP和LP线之间。在播种后11 ~ 17天，NP系的植酸酶和比植酸酶活性是LP系的1.3 ~ 2.6倍。LP系比NP系提前2天水解所有植酸。矿物的浓度和可提取性随着时间的推移而变化，在发芽过程中，LP线有较高的摩尔比的植酸钙，镁，钾比NP线。这些结果表明，种子中低植酸水平不会影响幼苗的萌发和生长。我们认为，LP线受益于更高的生物利用度的P和宏量矿物质不像NP线。
Phytate is a storage form of phosphorus (P) in seeds and, when degraded, plays a vital role in seed germination. Low phytate content in grain seeds inhibits germination and seedling growth. Here, Low-phytate (LP) and normal-phytate (NP) soybean lines were grown in vermiculite to determine whether germination was affected by low phytate content. Growth, phytase activity, mineral concentration, and mineral extractability from the seedlings of NP and LP soybean lines were evaluated. Seedling growth did not differ significantly between NP and LP lines. Phytase and specific phytase activities at 11 days to 17 days after sowing were 1.3 to 2.6 folds greater in the NP line than in the LP line. The LP line hydrolyzed all the phytate 2 days earlier than the NP line. The concentration and extractability of minerals changed over time during germination, and the LP line had higher molar ratios of phytic acid to Ca, Mg, and K than the NP line. These results suggest that germination and growth of the seedlings are not affected by low phytate levels in seeds. We suggest that the LP line benefits from higher bioavailability of P and macro-minerals unlike the NP line.