A microfluidic array with cellular valving for single cell co-culture
A microfluidic array with cellular valving for single cell co-culture
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DOI：
10.1039/c0lc00172d
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
West, Jonathan
中科院分区：
文献类型：
--
作者：
Frimat, Jean-Philippe;Becker, Marco;West, Jonathan
我们提出了一种高度平行的微流体接触单细胞对的方法。该方法将差分流体阻力捕获方法与用于同型和异型单细胞共培养的新型细胞阀门原理相结合。微分流体阻力用于顺序单细胞排列，其中微结构化环境内的活细胞的粘附和扁平化用于产生处于打开状态的阀。将流动的回流用于第二细胞类型的顺序单细胞阵列。需要沿着阻力最小的线性路径进行等离子体模板印刷以将细胞限制在捕获区域内。确定了具有最小剪切应力的预充流条件，用于高效细胞排列（类似于99%）和长期细胞培养。更大的陷阱尺寸使细胞配对的最高水平（类似于70%）。单细胞共培养物非常接近，用于连接子结构的形成和通讯接触模式的研究。该研究进一步强调了利用细胞的自然行为作为响应微流体元件背后的工作原理的可能性。
We present a highly parallel microfluidic approach for contacting single cell pairs. The approach combines a differential fluidic resistance trapping method with a novel cellular valving principle for homotypic and heterotypic single cell co-culturing. Differential fluidic resistance was used for sequential single cell arraying, with the adhesion and flattening of viable cells within the microstructured environment acting to produce valves in the open state. Reversal of the flow was used for the sequential single cell arraying of the second cell type. Plasma stencilling, along the linear path of least resistance, was required to confine the cells within the trap regions. Prime flow conditions with minimal shear stress were identified for highly efficient cell arraying (similar to 99%) and long term cell culture. Larger trap dimensions enabled the highest levels of cell pairing (similar to 70%). The single cell co-cultures were in close proximity for the formation of connexon structures and the study of contact modes of communication. The research further highlights the possibility of using the natural behaviour of cells as the working principle behind responsive microfluidic elements.