Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds
Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds
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DOI：
10.1016/j.bbaexp.2005.06.010
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发表时间：
2005-08-15
期刊：
影响因子：
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通讯作者：
Gautier, MF
中科院分区：
文献类型：
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作者：
Boutrot, F;Guirao, A;Gautier, MF
从普通小麦和硬粒小麦 cDNA 文库中分离出 9 个编码非特异性脂质转移蛋白 (nsLTP) 的 cDNA 克隆并进行表征。预计一种 cDNA 编码 2 型 nsLTP (7 kDa)，而其他 cDNA 编码 I 型 nsLTP (9 kDa)。所有编码的蛋白质均含有 N 端信号序列并具有 nsLTP 的特征。小麦nsLtp基因的基因组结构显示，2型TaLtp7.1a、TaLtp7.2a和1型TaLtp9.2b基因缺乏内含子，而其他1型基因则由一个内含子组成。禾本科 nsLTP 系统发育树的构建表明，小麦 nsLTP 可分为 11 个不同的类群，并且与大麦序列密切相关。利用逆转录聚合酶链反应（RT-PCR）分析，研究了小麦种子发育和发芽过程中九个 nsLtp 基因的表达模式。我们确定了 nsLtp 基因转录物积累的三种不同概况。 TdLtp7.1a、TdLtp9.4a 和 TdLtp9.7a 转录本在所有成熟阶段均检测到，而 TdLtP7.2a、TdLtP9.2a、TdLtp9.3a、TdLtp9.5a 和 TdLtp9.6a 转录本仅存在于种子发育的第一个阶段和 TdLtp9.1a 的最后阶段。而且，这9个小麦nsLtp基因不是种子特异性的，也在幼苗的胚芽鞘中表达。本研究揭示了小麦 nsLtp 基因家族的复杂性，并表明 nsLtp 基因的表达在种子中受到发育调节，表明每种相应蛋白质具有特定的功能。 (c) 2005 Elsevier B.V 保留所有权利。
Nine cDNA clones encoding non-specific lipid transfer proteins (nsLTPs) were isolated from Triticum aestivum and Triticum durum cDNA libraries and characterized. One cDNA is predicted to encode a type 2 nsLTP (7 kDa) while others encode type I nsLTPs (9 kDa). All encoded proteins contain an N-terminal signal sequence and possess the characteristic features of nsLTPs. The genomic structures of the wheat nsLtp genes show that type 2 TaLtp7.1a, TaLtp7.2a and type 1 TaLtp9.2b genes lack introns while the other type 1 genes consist of one intron. Construction of a phylogenic tree of Poaceae nsLTPs shows that wheat nsLTPs can be divided into eleven distinct groups and are closely related to barley sequences. Using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, the expression patterns of nine nsLtp genes were studied during wheat seed development and germination. We identified three different profiles of nsLtp gene transcript accumulation. Whereas TdLtp7.1a, TdLtp9.4a and TdLtp9.7a transcripts were detected during all maturation stages, TdLtP7.2a, TdLtP9.2a, TdLtp9.3a, TdLtp9.5a and TdLtp9.6a transcripts were only present in the first and TdLtp9.1a in the last stages of seed development. Moreover, these nine wheat nsLtp genes are not seed-specific and are also expressed in the coleoptile of young seedlings. The present study revealed the complexity of the wheat nsLtp gene family and showed that the expression of nsLtp genes is developmentally regulated in the seeds, suggesting a specific function for each of the corresponding proteins. (c) 2005 Elsevier B.V All rights reserved.