Dietary Capsaicin Ameliorates Pressure Overload-Induced Cardiac Hypertrophy and Fibrosis Through the Transient Receptor Potential Vanilloid Type 1
Dietary Capsaicin Ameliorates Pressure Overload-Induced Cardiac Hypertrophy and Fibrosis Through the Transient Receptor Potential Vanilloid Type 1
复制标题
膳食辣椒素通过瞬时受体电位香草酸1型改善压力超负荷引起的心脏肥大和纤维化。
DOI：
10.1093/ajh/hpu068
复制
发表时间：
2014-12-01
影响因子：
3.2
通讯作者：
Yang, Dachun
中科院分区：
文献类型：
--
作者：
Wang, Qiang;Ma, Shuangtao;Yang, Dachun
背景
膳食辣椒素通过激活非选择性阳离子通道瞬时受体电位香草酸1（TRPV 1），在高血压、动脉粥样硬化、肥胖和高脂血症中发挥保护作用。本研究旨在研究辣椒素在压力超负荷模型中心脏肥大和纤维化中的作用。
方法
将8周龄TRPV 1敲除（KO）小鼠及其野生型（WT）同窝仔随机分为假手术组和主动脉结扎手术组，并喂食饲料或饲料加辣椒素10周。
结果
膳食辣椒素显著减弱压力超负荷诱导的WT小鼠心脏重量指数增加、心室容积增大、心脏功能降低和心脏纤维化增加。然而，辣椒素的这些作用在TRPV 1 KO小鼠中不存在。此外，辣椒素减弱了WT小鼠中压力超负荷诱导的转化生长因子β、结缔组织生长因子和Smad 2/3磷酸化的上调，但在TRPV 1 KO小鼠中没有。此外，辣椒素减弱压力超负荷诱导的金属蛋白酶（MMP）-2，MMP-9和MMP-13在WT小鼠中的过度表达，但在TRPV 1 KO小鼠中没有。辣椒素也减弱了血管紧张素II诱导的TRPV 1通道小鼠心脏成纤维细胞增殖。
结论
我们的研究结果表明，饮食辣椒素通过TRPV 1保护压力超负荷小鼠的心脏肥大和纤维化。
BACKGROUND
Dietary capsaicin plays a protective role in hypertension, atherosclerosis, obesity, and hyperlipidemia through activating the transient receptor potential vanilloid type 1 (TRPV1), a nonselective cation channel. This study was designed to investigate the role of capsaicin in cardiac hypertrophy and fibrosis in a pressure overload model.
METHODS
TRPV1 knockout (KO) mice and their wild-type (WT) littermates, aged 8 weeks, were randomly divided into sham and aortic banding surgery groups and were fed with chow or chow plus capsaicin for 10 weeks.
RESULTS
Dietary capsaicin significantly attenuates pressure overload-induced increase in heart weight index, enlargement of ventricular volume, decrease in cardiac function, and increase in cardiac fibrosis in WT mice. However, these effects of capsaicin were absent in TRPV1 KO mice. Additionally, capsaicin blunted pressure overload-induced upregulation of transforming growth factor β, connective tissue growth factor, and the phosphorylation of Smad2/3 in WT mice but not in TRPV1 KO mice. Moreover, capsaicin attenuated pressure overload-induced overexpression of metalloproteinase (MMP)-2, MMP-9 and MMP-13 in WT mice but not in TRPV1 KO mice. Capsaicin also attenuated angiotensin II-induced proliferation of cardiac fibroblasts from mice with the TRPV1 channel.
CONCLUSIONS
Our results suggest that dietary capsaicin protects against cardiac hypertrophy and fibrosis in pressure overload mice through TRPV1.