Exposure to dilute concentrations of bupropion affects zebrafish early life stages
Exposure to dilute concentrations of bupropion affects zebrafish early life stages
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DOI：
10.1016/j.chemosphere.2019.01.141
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发表时间：
2019-05-01
期刊：
影响因子：
8.8
通讯作者：
Grisolia, Cesar Koppe
中科院分区：
文献类型：
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作者：
Franco, Mariana Bernardes;Andrade, Thayres S.;Grisolia, Cesar Koppe
精神药物是全球处方最多的活性物质之一。安非他酮（BPP）是一种抗抑郁药，通过抑制去甲肾上腺素和多巴胺再吸收发挥作用。在各种水体基质中都发现了它，因此必须研究它对水生生物的影响。本研究旨在评价BPP对斑马鱼（Danio rerio）早期生命阶段的急性毒性效应。对于发育分析，将生物体暴露于0至82000 μ g/L的浓度范围内168 h。通过将胚胎暴露于广泛的浓度范围（从0到50000 μ g/L）来进行另外两个实验，以评估BPP对胚胎行为的影响，使用Zebrabox并在生化水平上进行测试（乙酰胆碱酯酶，谷胱甘肽-S-转移酶，乳酸脱氢酶和过氧化氢酶）。发育分析表明，BPP具有较低的急性毒性，计算的168 h-LC 50为50346 μ g/L。浓度等于或高于44800微克/升引起了几种影响，如孵化延迟，水肿和尾巴畸形。然而，从7300微克/升以上的浓度引起平衡改变。行为学分析表明，BPP影响斑马鱼的运动行为，在0.6 μ g/L时降低活动，在8.8和158 μ g/L时增加活动，在50000 μ g/L时降低活动。生化分析表明，在158和2812 μ g/L的AChE活性增加，GST的增加在最高浓度，CAT的改变和LDH的增加在0.6，2812和50000 μ g/L。我们可以得出结论，BPP影响斑马鱼早期生命阶段的环境浓度。(C)2019爱思唯尔有限公司版权所有。
Psychiatric pharmaceuticals are one of the most prescribed active substances globally. Bupropion (BPP) is an antidepressant that acts via inhibition of norepinephrine and dopamine reuptake. It has been found in various water matrices, and thus its effects on aquatic organisms must be studied. The present study aimed to evaluate the acute toxic effects of BPP on zebrafish (Danio rerio) early life stages. For developmental analysis, organisms were exposed for 168 h to concentrations ranging from 0 to 82000 mu g/L. Two other experiments were performed by exposing embryos to a wide range of concentrations (from 0 to 50000 mu g/L) in order to evaluate BPP effects on embryonic behavior, using the Zebrabox and testing at the biochemical level (acetylcholinesterase, glutathione-S-transferase, lactate dehydrogenase and catalase). Developmental analysis indicated that BPP had low acute toxicity with a calculated 168 h-LC50 of 50346 mu g/L. Concentrations equal to or above 44800 mu g/L elicited several effects such as hatching delay, edemas and tail deformities. However, concentrations from 7300 mu g/L upwards elicited equilibrium alteration. Behavioral analysis showed that BPP affected zebrafish locomotor behavior by decreasing activity at 0.6 mu g/L, increasing activity at 8.8 and 158 mu g/L, and decreasing activity at 50000 mu g/L. Biochemical analysis showed an increase of AChE activity at 158 and 2812 mu g/L, an increase in GST at the highest concentrations, CAT alteration and increase of LDH at 0.6, 2812 and 50000 mu g/L. We can conclude that BPP affects zebrafish early life stages at environmental concentrations. (C) 2019 Elsevier Ltd. All rights reserved.