Amniotic fluid volume and composition in mouse pregnancy
Amniotic fluid volume and composition in mouse pregnancy
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DOI：
10.1016/j.jsgi.2005.08.008
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发表时间：
2005-12-01
期刊：
影响因子：
--
通讯作者：
Brace, RA
中科院分区：
文献类型：
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作者：
Cheung, CY;Brace, RA
目的：本研究旨在确定怀孕小鼠妊娠期间羊水 (AF) 体积和成分的同步变化。方法：将 CB6F1 品系的年轻成年小鼠（6 至 7 周龄）交配过夜。在胚胎前第 9.5 天至第 18.5 天连续收集 AF，以测量体积和成分。统计分析包括单因素方差分析 (ANOVA)。 结果：AF 体积从第 9.5 天的 18 +/- 4 (SE) μL 增加到第 15.5 天至 16.5 天的最大值 147 +/- 4 μL，并在第 18.5 天急剧下降至 17 +/- 3 μL。除了在第 19.5 天分娩前升高至 20.5 之外，AF 渗透压没有变化。随着妊娠的进展，AF 钠、钙和葡萄糖浓度先升高后降低。 AF 钾、氯和乳酸浓度在整个妊娠期间最初下降，然后上升。在第 9.5 天之前和第 18.5 天之后，AF 体积太小，无法进行体积或成分测定。结论：在小鼠中，AF 体积从妊娠中期到妊娠晚期最大的上升与人类相似，而分娩前的急剧下降则不然。正如在胎羊中观察到的，液体体积的变化与 AF 渗透压和溶质浓度的变化相关，而溶质浓度的变化与胎龄的增加相关。这些观察结果以及量化小鼠胎儿中 AF 体积和成分的可行性证明了使用基因改造小鼠作为未来研究 AF 体积和成分调节分子机制的模型的可能性。
OBJECTIVE: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse.METHODS: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days front embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA).RESULTS: AF volume increased from 18 +/- 4 (SE) mu L on day 9.5 to a maximum of 147 +/- 4 mu L on days 15.5 to 16.5 and decreased sharply to 17 +/- 3 mu L on day 18.5. AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5. AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and lactate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations.CONCLUSIONS: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume arc associated with AF osmolality and solute concentration changes that arc correlated with advancing gestational age. These observations together with the feasibility of quantifying AF volume and composition in the mouse fetus demonstrate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AF volume and composition.