Rheumatoid synovial cell morphologic changes induced by a mononuclear cell factor in culture.
Rheumatoid synovial cell morphologic changes induced by a mononuclear cell factor in culture.
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培养中单核细胞因子诱导的类风湿滑膜细胞形态变化。
DOI：
10.1002/art.1780260102
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发表时间：
1983
影响因子：
--
通讯作者：
Krane,SM
中科院分区：
文献类型：
--
作者：
Baker,DG;Dayer,JM;Roelke,M;Schumacher,HR;Krane,SM
培养的贴壁类风湿性滑膜细胞产生大量的前列腺素E2（PGE 2）和胶原酶。当暴露于单核细胞衍生因子时，这些细胞表现出PGE 2和胶原酶产生的显著增加。此外，细胞形态变得更加星状。在该因子存在的情况下，吲哚美辛抑制PGE 2的产生和星状变化，而胶原酶的产生通常以高速率持续。向培养物中添加PGE 2可重现星状变化，环磷酸腺苷（cAMP）类似物8-溴-cAMP也是如此。秋水仙素抑制单核细胞衍生因子诱导的形态转化，而细胞松弛素B则无此作用。星状形态似乎依赖于PGE 2诱导的cAMP刺激，与胶原酶产生本身无关。
Adherent rheumatoid synovial cells in culture produce large amounts of prostaglandin E2(PGE2) and collagenase. When exposed to a monocyte‐derived factor, such cells exhibit marked increases in PGE2and collagenase production. In addition, cellular morphology becomes more stellate. In the presence of this factor, indomethacin inhibits both PGE2production and the stellate changes, whereas collagenase production usually continues at a high rate. Addition of PGE2to cultures reproduces the stellate change as does the cyclic adenosine monophosphate (cAMP) analog 8‐bromo‐cAMP. Colchicine inhibits morphologic transformation induced by the monocyte‐derived factor, whereas cytochalasin B has no effect. It appears that the stellate morphology is dependent upon PGE2‐induced cAMP stimulation and is not related to collagenase production per se.