GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis
GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis
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DOI：
10.1152/ajpgi.2000.279.6.g1249
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发表时间：
2000-12-01
影响因子：
4.5
通讯作者：
Sangild, PT
中科院分区：
文献类型：
--
作者：
Burrin, DG;Stoll, B;Sangild, PT
我们希望确定外源性胰高血糖素样肽(GLP)-2输注是否刺激肠外喂养的未成熟猪的肠道生长。仔猪（妊娠106 ~ 108 d）给予肠外营养物质输注（TPN）， TPN +人GLP-2 （25 nmol.kg(-1)）。(1)天)，或肠内母猪乳（ENT） 6天。注射[1-C-13]苯丙氨酸后，在体内测量肠道蛋白质合成，并根据蛋白质积累和合成之间的差异计算降解。采用5-溴脱氧尿苷（BrdU）和末端dUTP镍端标记（TUNEL）原位检测隐窝细胞增殖和凋亡。GLP-2组和ENT组的肠道蛋白和DNA增加率及绒毛高度相似，且均高于TPN组（P < 0.05）。与TPN猪相比，GLP-2降低了部分蛋白质降解率，而ENT提高了部分蛋白质合成率。GLP-2组和ENT组tunel阳性细胞比例分别比TPN组低48%和64% （P, 0.05）。然而，与TPN仔猪相比，ENT（而非GLP-2）增加了brdu阳性隐窝细胞的百分比。我们得出结论，GLP-2通过减少蛋白质水解和细胞凋亡来促进早产儿tpn喂养猪的肠道生长，而肠内营养通过增加蛋白质合成和细胞增殖以及减少细胞凋亡来发挥作用。
We wished to determine whether exogenous glucagon-like peptide (GLP)-2 infusion stimulates intestinal growth in parenterally fed immature pigs. Piglets (106-108 days gestation) were given parenteral nutrient infusion (TPN), TPN + human GLP-2 (25 nmol.kg(-1).day(-1)), or sow's milk enterally (ENT) for 6 days. Intestinal protein synthesis was then measured in vivo after a bolus dose of [1-C-13] phenylalanine, and degradation was calculated from the difference between protein accretion and synthesis. Crypt cell proliferation and apoptosis were measured in situ by 5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end labeling (TUNEL), respectively. Intestinal protein and DNA accretion rates and villus heights were similar in GLP-2 and ENT pigs, and both were higher (P < 0.05) than in TPN pigs. GLP-2 decreased fractional protein degradation rate, whereas ENT increased fractional protein synthesis rate compared with TPN pigs. Percentage of TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64% lower, respectively, than in TPN group (P, 0.05). However, ENT, but not GLP-2, increased percentage of BrdU-positive crypt cells above that in TPN piglets. We conclude that GLP-2 increases intestinal growth in premature, TPN-fed pigs by decreasing proteolysis and apoptosis, whereas enteral nutrition acts via increased protein synthesis and cell proliferation and decreased apoptosis.