Salmonella typhimurium infection in calves: specific immune reactivity against O-antigenic polysaccharide detectable in in vitro assays
Salmonella typhimurium infection in calves: specific immune reactivity against O-antigenic polysaccharide detectable in in vitro assays
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犊牛鼠伤寒沙门氏菌感染：体外检测可检测到针对 O 抗原多糖的特异性免疫反应
DOI：
10.1128/iai.37.2.728-736.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
A. Lindberg
中科院分区：
文献类型：
--
作者：
J. Robertsson;C. Fossum;S. Svenson;A. Lindberg
收集鼠伤寒沙门菌（O抗原4,5,12）或都柏林沙门菌血清型（O抗原9,12）感染犊牛的外周血淋巴细胞，用不同的细菌包膜成分刺激其[3H]胸腺嘧啶掺入量。结果发现，与未感染犊牛的外周血淋巴细胞相比，感染犊牛外周血淋巴细胞中[3H]胸苷的含量显著增加（P值范围小于0.05 ~小于0.0005）。应答细胞类型是在b细胞缺失和t细胞富集的人群中发现的。沙门氏菌感染引发了t细胞对至少两种细胞包膜成分的反应：(i)针对脂多糖的O抗原多糖链的特异性反应(这一点很明显，来自肠炎沙门氏菌[O 9,12]的多糖与鼠伤寒沙门氏菌具有三糖结构[O抗原12决定因素]，刺激了[3H]胸腺嘧啶的摄取，尽管比同源系统低，(2)对鼠伤寒沙门氏菌和都柏林沙门氏菌血清型中均存在的外膜蛋白（孔蛋白）产生应答。用血清型dublin沙门氏菌感染犊牛外周血淋巴细胞进行的实验结果与鼠伤寒沙门氏菌感染犊牛的结果非常吻合。
Peripheral blood lymphocytes collected from calves infected experimentally with Salmonella typhimurium (O antigens 4,5,12) or Salmonella sp. serotype dublin (O 9,12) were stimulated with various bacterial cell envelope components, and their [3H]thymidine incorporation was measured. It was found that peripheral blood lymphocytes from infected calves incorporated significantly more [3H]thymidine than peripheral blood lymphocytes from uninfected controls (P values ranged from less than 0.05 to less than 0.0005). The responder cell type was found in a B-cell-depleted and T-cell-enriched population. The Salmonella infections elicited T-cell responses against at least two cell envelope components: (i) a specific response against the O-antigenic polysaccharide chain of the lipopolysaccharide (This was evident in that a polysaccharide from S. enteritidis [O 9,12] which shares a trisaccharide structure [O antigen 12 determinant] with S. typhimurium stimulated [3H]thymidine uptake, which, although lower than in the homologous system, was significantly higher than that seen after incubation with unrelated Salmonella sp serotype thompson polysaccharide.) and (ii) a response against outer membrane proteins (porins), which are present in both S. typhimurium and Salmonella sp. serotype dublin. The experiments with peripheral blood lymphocytes from Salmonella sp. serotype dublin-infected calves gave results in excellent agreement with those obtained in S. typhimurium-infected calves.