[Development of Dasypyrum genome specific marker by using wheat microsatellites].
[Development of Dasypyrum genome specific marker by using wheat microsatellites].
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DOI：
10.1360/yc-006-1573
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发表时间：
2006-12
期刊：
影响因子：
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通讯作者：
Cheng Liu;Zujun Yang;Juan Feng;Jianping Zhou;Shi-Hua Chi;Z. Ren
中科院分区：
文献类型：
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作者：
Cheng Liu;Zujun Yang;Juan Feng;Jianping Zhou;Shi-Hua Chi;Z. Ren
以普通小麦中国春和优良小麦品种为对照，对分布于普通小麦1A ~ 7A、1B ~ 7 B、1D ~ 7 D染色体上的102对SSR引物，在短芒簇毛麦、簇毛麦、小麦-簇毛麦双二倍体及其衍生物上进行了分析。引物Xgwm 301在所有含簇毛麦染色体的材料中均扩增出约400 bp（测序长度为415 bp，命名为Xgwm 301/415）的特异多态性DNA片段，但在供试普通小麦中没有扩增出。此外，对一组T. aestival-Dasypyrum的附加染色体，结果表明，这7对绒毛染色体均含有Xgwm 301/415。因此，Xgwm 301/415是簇毛麦属的一个基因组特异多态性片段，可作为小麦簇毛麦属染色体检测的分子标记。
One hundred and two SSR primer pairs, distributed in chromosome 1A to 7A, 1B to 7B, 1D to 7D of Triticum aestivum, were investigated on Dasypyrum breviaristatum, D.villosum, wheat-Dasypyrum amphiploids and its derivatives, with the control of common wheat Chinese Spring and elite wheat cultivars. A specific polymorphic DNA fragment of about 400 bp (415 bp-long by sequenced, named Xgwm301/415) amplified by primer pair Xgwm301 was obtained in all lines containing Dasypyrum chromosomes, but there were not the case in the tested common wheat. Furthermore, PCR analysis was performed on a set of T. aestivum-Dasypyrum addition, the result showed that all the seven pairs of villosum chromosomes contain Xgwm301/415. Therefore, Xgwm301/415 is a genome-specific polymorphic DNA segment for genera of Dasypyrum, and it could be used as a molecular marker for detection of chromosomes of Dasypyrum in wheat.