Morphological and molecular characterization of Aphelenchoides fujianensis n. sp. (Nematoda: Aphelenchoididae) from Pinus massoniana in China.
Morphological and molecular characterization of Aphelenchoides fujianensis n. sp. (Nematoda: Aphelenchoididae) from Pinus massoniana in China.
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DOI：
10.11646/zootaxa.2509.1.3
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发表时间：
2010-06
期刊：
影响因子：
0.9
通讯作者：
K. Zhuo;R. Cui;W. Ye;M. Luo;Honghong Wang;Hu Xue-nan;J. Liao
中科院分区：
文献类型：
--
作者：
K. Zhuo;R. Cui;W. Ye;M. Luo;Honghong Wang;Hu Xue-nan;J. Liao
福建小菜蛾Aphelenchoidesfujianensis n.sp.根据线粒体DNA的近全长小亚基rDNA基因和部分细胞色素氧化酶亚基I的形态和分子分析，描述并图解了一株死亡的马尾松。这一新种属于星形尾端的Aphelenchoidae Sensu Shahina组3，其特征是身体较长(雄性为653-843μm，雌性为803-941μm)，在侧场有四个侧切齿。雄性有较大的针状体(24-30μm)。雌性外阴后有细长的子宫囊(外阴-肛门距离的32-44%)，通常带有精子。雄性和雌性都有星形的短尖。它与其他物种的区别在于外阴后子宫囊的长度、a和c的比例以及毛刺的大小和形状。分子分析表明，该种具有独特的18S和mt-DNA序列，并且在使用这两个标记推断的树状图上与Aphelenchoides Besseyi最接近。福建黄曲霉OEPP/EPPO的识别代码。分别是：A1-B2-C1-D1/3-E1-F1/2。
Aphelenchoides fujianensis n. sp. is described and illustrated from a dead Pinus massoniana based on morphology and molecular analyses of the near-full-length small subunit rDNA gene and partial cytochrome oxidase subunit I of mitochondrial DNA. This new species belongs to the Group 3 of Aphelenchoides species sensu Shahina with star-shaped tail terminus and is characterised by a relatively long body (653–843 μ m in the male and 803–941 μ m in the female) and four lateral incisures in the lateral field. The male has relatively large spicules (24–30 μ m). The female has elongate postvulval uterine sac (extending ca 32–44% of vulva-anus distance), usually with sperms. Both male and female have star-shaped mucro. It is distinguished from other species by postvulval uterine sac length, a and c ratios, and spicule size and shape. Molecular analysis reveals that this species has unique 18S and mt-DNA sequences, and is closest to Aphelenchoides besseyi in dendrograms inferred using both markers. The identification codes of OEPP/EPPO for A. fujianensis n. sp. are: A1-B2-C1-D1/3-E1-F1/2.