The source of human mesenchymal stromal cells influences their TLR profile as well as their functional properties
The source of human mesenchymal stromal cells influences their TLR profile as well as their functional properties
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DOI：
10.1016/j.cellimm.2011.05.010
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发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Lagneaux, Laurence
中科院分区：
文献类型：
--
作者：
Raicevic, Gordana;Najar, Mehdi;Lagneaux, Laurence
间充质基质细胞（MSC）可以从不同来源扩增。我们比较了炎症和TLR连接对来自骨髓（BM）、脂肪组织（AT）和沃顿氏胶（WJ）的MSC的表型和功能的影响。WJ-MSC的特征在于缺乏TLR 4表达。虽然炎症上调了所有三种MSC类型中的TLR 3，但仅在BM-MSC上观察到TLR 4上调。TLR连接增加BM-和AT-MSC中炎性细胞因子的产生，但不增加WJ-MSC中的炎性细胞因子，并增加AT-MSC中的抗炎细胞因子。尽管炎症增加了所有MSC类型的炎性细胞因子的分泌，但额外的TLR触发对WJ-MSC没有进一步的影响。WJ-MSC对MLR的免疫抑制潜力既不受炎症的影响，也不受TLR触发的影响。这种耐药性与HGF的过度产生有关。这些数据表明，MSC来源可能是重要的，而设计免疫调节细胞移植治疗。(C)2011 Elsevier Inc. All rights reserved.
Mesenchymal stromal cells (MSC) can be expanded from different sources. We compared the influence of inflammation and TLR ligation on the phenotype and function of MSC derived from bone marrow (BM), adipose tissue (AT), and Wharton's jelly (WJ). WJ-MSC were featured by a lack of TLR4 expression. While inflammation upregulated TLR3 in all three MSC types, TLR4 upregulation was observed only on BM-MSC. TLR ligation increased the production of inflammatory cytokines in BM- and AT-MSC but not in WJ-MSC and augmented anti-inflammatory cytokines in AT-MSC. Although inflammation increased in all MSC types the secretion of inflammatory cytokines, additional TLR triggering did not have further effect on WJ-MSC. The immunosuppressive potential of WJ-MSC on MLR was affected neither by inflammation nor by TLR triggering. This resistance was related to an overproduction of HGF. These data indicate that MSC source could be of importance while designing immunomodulating cell therapy in transplantation. (C) 2011 Elsevier Inc. All rights reserved.