Specific radiation damage can be used to solve macromolecular crystal structures
Specific radiation damage can be used to solve macromolecular crystal structures
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DOI：
10.1016/s0969-2126(03)00006-6
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发表时间：
2003-02-01
期刊：
影响因子：
5.7
通讯作者：
McSweeney, S
中科院分区：
文献类型：
--
作者：
Ravelli, RBG;Leiros, HKS;McSweeney, S
第三代同步加速器源的使用引起了人们对电离辐射对晶体生物样品影响的新关注。一般来说，这一问题被视为应该避免的问题。然而，本文表明，辐射损伤远非成功确定结构的障碍，而是为大分子结构定相提供了机会。这在蛋白质和寡核苷酸上都得到了成功的证明，通过它们自动构建了完整的模型。讨论了通过利用辐射损伤，相位问题可能成为大分子晶体结构测定的障碍的可能性。
The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, byway of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.