Stimulation of Titanium Implant Osseointegration Through High-Frequency Vibration Loading is Enhanced when Applied at High Acceleration
Stimulation of Titanium Implant Osseointegration Through High-Frequency Vibration Loading is Enhanced when Applied at High Acceleration
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DOI：
10.1007/s00223-014-9896-x
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发表时间：
2014-11-01
影响因子：
4.2
通讯作者：
Duyck, Joke
中科院分区：
文献类型：
--
作者：
Ogawa, Toru;Vandamme, Katleen;Duyck, Joke
通过全身振动（WBV）施加的低幅度高频载荷会影响骨骼。本研究的目的是将WBV载荷刺激分解为其组成元素，并分别研究频率和加速度对钛种植体周围骨组织动力学的影响。在120只大鼠的胫骨中插入钛植入物。将大鼠分为1个对照组（无负载）和5个低（L）、中（M）或高（H）频率范围和加速度试验组[0.3xg时12-30 Hz（F（L）A（H））; 0.075xg时70-90 Hz（F（M）A（M））; 0.3xg时70-90 Hz（F（M）A（H））; 130-150 Hz，0.043xg（F（H）A（L））; 130-150 Hz，0.3xg（F（H）A（H））]。WBV应用1或4周。通过定量组织学（骨-种植体接触（BIC）和种植体周围骨形成（BV/TV））评价种植体骨整合。进行双因素ANOVA（实验期持续时间;加载模式），α = 0.05。BIC随时间和负荷显著增加（p < 0.0001）。在具有中等或高频率的高加速度下的加载方案中发现最高BIC（F（M）A（H）和F（H）A（H）），并且与F（L）A（H）和F（M）A（M）显著不同（分别为p < 0.02和p < 0.005）。BV/TV随时间显著降低（p < 0.0001）。负荷导致位点特异性BV/TV增加（p < 0.001）。F（M）A（H）和F（H）A（H）的BV/TV反应最高，与F（M）A（M）显著不同（p < 0.005）。研究结果揭示了高频振动载荷加速和增强种植体骨整合的潜力，特别是在高加速度下应用时。这种机械信号具有很大的潜力，但尚未开发，可用作非药物治疗，以改善受损骨中的种植体骨整合。
Low-magnitude high-frequency loading, applied by means of whole body vibration (WBV), affects the bone. Deconstructing a WBV loading stimulus into its constituent elements and investigating the effects of frequency and acceleration individually on bone tissue kinetics around titanium implants were aimed for in this study. A titanium implant was inserted in the tibia of 120 rats. The rats were divided into 1 control group (no loading) and 5 test groups with low (L), medium (M) or high (H) frequency ranges and accelerations [12-30 Hz at 0.3xg (F (L) A (H)); 70-90 Hz at 0.075xg (F (M) A (M)); 70-90 Hz at 0.3xg (F (M) A (H)); 130-150 Hz at 0.043xg (F (H) A (L)); 130-150 Hz at 0.3xg (F (H) A (H))]. WBV was applied for 1 or 4 weeks. Implant osseointegration was evaluated by quantitative histology (bone-to-implant contact (BIC) and peri-implant bone formation (BV/TV)). A 2-way ANOVA (duration of experimental period; loading mode) with alpha = 0.05 was performed. BIC significantly increased over time and under load (p < 0.0001). The highest BICs were found for loading regimes at high acceleration with medium or high frequency (F (M) A (H) and F (H) A (H)), and significantly differing from F (L) A (H) and F (M) A (M) (p < 0.02 and p < 0.005 respectively). BV/TV significantly decreased over time (p < 0.0001). Loading led to a site-specific BV/TV increase (p < 0.001). The highest BV/TV responses were found for F (M) A (H) and F (H) A (H), significantly differing from F (M) A (M) (p < 0.005). The findings reveal the potential of high-frequency vibration loading to accelerate and enhance implant osseointegration, in particular when applied at high acceleration. Such mechanical signals hold great, though untapped, potential to be used as non-pharmacologic treatment for improving implant osseointegration in compromised bone.