Generating Monocyte-Derived Endothelial-like Cells for Vascular Regeneration.
Generating Monocyte-Derived Endothelial-like Cells for Vascular Regeneration.
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DOI：
10.1007/978-1-0716-1708-3_2
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发表时间：
2022
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工程血管移植的一个主要限制是缺乏适当的内皮来防止血栓形成和随后的移植失败。从患者血管中获取内皮细胞是侵入性的，需要大量的培养时间。在这里，我们提出了一种替代策略，即从外周血中培养丰富且易于获取的单核细胞，并将其分化为内皮样状态，能够通过产生一氧化氮和形成内皮粘附连接来预防血栓形成。考虑到外周血中存在过多的单核细胞，这种方法为生成血管移植物所需的内皮细胞提供了一种强大的替代方法。
A major limitation in engineering vascular grafts is the lack of proper endothelium to prevent thrombosis and subsequent graft failure. Obtaining endothelial cells from patients’ vasculature is intrusive and requires extensive culture time. Here we present an alternative strategy wherein abundant and easily accessible monocytes from peripheral blood are cultured and differentiated towards an endothelial like state capable of preventing thrombosis through production of nitric oxide and formation of endothelial adherens junctions. Considering the plethora of monocytes present within peripheral blood, this method provides a robust alternative to generating endothelial cells required for vascular graft production.