Understanding the Microbiological Quality of Drinking Water at the Point of Consumption with Citizen Science
Understanding the Microbiological Quality of Drinking Water at the Point of Consumption with Citizen Science
复制标题
DOI：
10.1021/acsestwater.3c00184
复制
发表时间：
2023-07
期刊：
影响因子：
--
通讯作者：
Bo Li;Xingnan Li;Prakit Saingam;T. Yan
中科院分区：
文献类型：
--
作者：
Bo Li;Xingnan Li;Prakit Saingam;T. Yan
在消费者消费点进行水质监测可以提高对饮用水微生物风险的认识。在这项研究中，饮用水样本收集公民科学参与者根据他们的正常饮用水源和行为。在六种不同的样本类型中，自来水在采样频率（39%）和加权重要性得分方面受到最多关注，其次是饮水机（38%）和个人容器（21%）。作为指示生物的大肠杆菌和总大肠菌群的计数在自来水和饮水机的水样中表现出一致和良好的质量，但在个人容器的水样中检测频率（分别为90%和90%）和中值浓度（分别为14和252 CFU/100 mL）显著较高。基于16 S rRNA基因扩增子测序的微生物群落分析表明，个人容器水样中的微生物群落多样性低于其他类型水样中的微生物群落，后者在微生物组成和α和β多样性方面表现出较高的相似性。几个属，包括物种的机会致病菌检测到跨样品从饮用水源，而较高的总相对丰度，这些属的水样品中检测到个人容器比其他。
Water quality monitoring at the point of consumption by consumers can improve the understanding of the microbial risk of drinking water. In this study, drinking water samples were collected by citizen science participants according to their normal drinking sources and behaviors. Among six different sample types, tap water received the most attention per sampling frequency (39%) and weighted importance score, followed by drinking fountain (38%) and personal container (21%). Enumeration ofEscherichia coliand the total coliform as indicator organisms showed consistent and good quality in water samples from tap water and drinking fountains but significantly higher detection frequencies (90 and 90%, respectively) and median concentrations (14 and 252 CFU/100 mL, respectively) in water samples from personal containers. Microbial community analysis based on 16S rRNA gene amplicon sequencing showed that the microbial communities in water samples from personal containers were less diverse than microbial communities in the other water types, the latter of which showed high similarity in the microbial composition and α and β diversity. Several genera that include species of opportunistic pathogens were detected across samples from drinking water sources, while higher total relative abundances of these genera were detected in water samples from personal containers than others.