The anti-atherosclerotic effects of puerarin on induced-atherosclerosis in rabbits
The anti-atherosclerotic effects of puerarin on induced-atherosclerosis in rabbits
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DOI：
10.5507/bp.2013.096
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发表时间：
2015-01-01
影响因子：
0.9
通讯作者：
Agula, B.
中科院分区：
文献类型：
--
作者：
Bao, Lidao;Zhang, Ying;Agula, B.
背景。心血管疾病是世界范围内的一个主要健康问题，传统草药提取物可以很好地治疗心血管疾病。葛根，豆科植物葛根的干燥根（野生）。梧桐是中国南方一些省份的一种美味蔬菜。葛根素也被广泛用于治疗人类疾病，但很少有对照研究。探讨葛根素对脂肪性动脉粥样硬化（AS）的抗动脉粥样硬化作用。采用高脂饲料喂养60只家兔建立AS模型，随机分为6组：(1)正常对照组，(2)模型组(3)他汀、辛伐他汀组，(4)、(5)、(6)组分别给予3种不同剂量的葛根素。在治疗前和治疗后90天收集各组动物的空腹血清，测定总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白-胆固醇（LDL-C）和高密度脂蛋白-胆固醇（HDL-C）水平。对主动脉进行病理形态学检查。Western blot检测PCNA和PDGF-A蛋白水平。第90天，葛根素高、中剂量组和辛伐他汀组大鼠TC、TG、LDL-C水平均显著低于模型组(P
Background. Cardiovascular disease is a major health issue worldwide, which has been well treated by the extracts of traditional herbs. Radix Puerariae, the dried root of the leguminous plant Pueraria lobata (Willd.) Ohwi, is a delicious vegetable in some southern provinces of China. Puerarin has also been widely used to treat human diseases, but few controlled studies are available.Aim. To determine the anti-atherosclerotic effects of puerarin on fat diet-induced atherosclerosis (AS) in rabbits.Methods. An AS model was established by feeding 60 rabbits a high-fat diet and randomly dividing them into 6 groups: (1) normal control, (2) a model group (3) the statin, simvastatin and groups (4), (5) and (6) received 3 different amounts of puerarin. The fasting sera of all animals were collected before and after 90 days treatment to determine the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol (HDL-C). The aortas were pathomorphologically examined. PCNA and PDGF-A protein levels were assessed by Western blot.Results. On the 90th day, the levels of TC, TG and LDL-C were significantly lower in high-and middle-dose puerarin groups and simvastatin group than in the model group (P