Low Frequency Loss of Heterozygosity in the BRCA1 Region in Japanese Sporadic Breast Cancer.
Low Frequency Loss of Heterozygosity in the BRCA1 Region in Japanese Sporadic Breast Cancer.
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DOI：
10.1007/bf02966980
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发表时间：
1996-12-20
期刊：
影响因子：
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通讯作者：
Kobayashi
中科院分区：
文献类型：
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作者：
Yamashita;Iwase;Kobayashi
为了研究BRCA 1基因在日本散发性乳腺癌中的可能参与，我们使用5个微卫星标记，如D17 S250、D17 S846、D17 S855、D17 S579和NME 1，分析了101例散发性乳腺癌BRCA 1区域（17 q12-q22）的杂合性丢失（洛）。各标记的洛缺失率分别为14.9%（11/74）、11.9%（8/67）、14.3%（12/84）、5.3%（4/75）和13.3%（9/68）。5个标志物中至少有1个发生洛缺失的发生率为23.8%（24/101）。BRCA 1区域的洛缺失与临床病理因素无关。尽管使用显微切割方法，我们的系列中的洛缺失频率相对较低，特别是在西方国家。我们认为，BRCA 1基因可能只发挥有限的作用，作为一个肿瘤抑制基因在大多数日本散发性乳腺癌。
To investigate the possible involvement of the BRCA1 gene in Japanese sporadic breast cancer we have analyzed loss of heterozygosity (LOH) at the BRCA1 region (17q12-q22) in 101 sporadic breast cancers using 5 microsatellite markers, such as D17S250, D17S846, D17S855, D17S579, and NME1. The frequency of LOH in each markers was 14.9%(11/74), 11.9 %(8/67),14.3%(12/84),5.3%(4/75), and13.3%(9/68),respectively. The incidence of LOH in at least one of 5 markers was 23.8%(24/101). The LOH at the BRCA1 region did not significantly correlate with clinicopathologic factors. Despite using a microdissection method, the LOH frequency in our series was relatively lower than other reports, especially in Western countries. We suggest that the BRCA1 gene might play only a limited role as a tumor suppressor gene in the majority of Japanese sporadic breast cancers.