Effects of Controlled Released BMP-7 on Markers of Inflammation and Degradation During the Cultivation of Human Osteoarthritic Chondrocytes
Effects of Controlled Released BMP-7 on Markers of Inflammation and Degradation During the Cultivation of Human Osteoarthritic Chondrocytes
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DOI：
10.1177/0885328210374671
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发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Schmidt-Rohlfing, Bernhard
中科院分区：
文献类型：
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作者：
Gavenis, Karsten;Pufe, Thomas;Schmidt-Rohlfing, Bernhard
本研究的目的是调查BMP-7释放聚乳酸微球的外观上的各种分解代谢和炎症细胞因子分泌的骨关节炎软骨细胞培养在胶原凝胶的影响。将患有骨关节炎的15名患者的关节软骨细胞转移到I型胶原凝胶中。另外，加入包封到聚丙交酯微球中的BMP-7（50 ng BMP-7/mL凝胶）。14天后，通过免疫组织化学染色和RT-PCR研究参与基质周转和炎症的各种基因的基因表达和蛋白质外观，并与未处理的对照进行比较。TNF-α、MMP-13、IL-6、IL-1 β和VEGF基因表达在治疗组中降低。相反，BMP-7诱导的基质合成不受影响，使II型胶原（Col-II）基因表达升高，而I型胶原（Col-I）降低。总之，低浓度BMP-7从聚乳酸微球中的受控释放导致所研究的炎症和基质降解标志物的基因表达降低，而基质合成被诱导。
The aim of the present study is to investigate the effects of BMP-7 released from polylactide microspheres on the appearance of various catabolic and inflammatory cytokines secreted by osteoarthritic chondrocytes cultivated in a collagen gel. Articular chondrocytes of 15 patients suffering from osteoarthritis are transferred to a collagen type-I gel. Additionally, BMP-7 encapsulated into polylactide microspheres (50 ng BMP-7/mL gel) is added. After 14 days, gene expression and protein appearance of various genes involved in matrix turnover and inflammation are investigated by immunohistochemical staining and RT-PCR and compared to untreated controls. TNF-alpha, MMP-13, IL-6, IL-1 beta, and VEGF gene expressions are decreased in the treatment group. In contrast, BMP-7-induced matrix synthesis is not affected, leaving collagen type-II (Col-II) gene expression to be elevated, while collagen type-I (Col-I) is decreased. In summary, controlled release of low concentrated BMP-7 from polylactide microspheres leads to a decrease in gene expression of the investigated inflammation and matrix degradation markers whereas matrix synthesis is induced.