The Symbiotic Bacteria Nardonella in Rice Water Weevil (Coleoptera: Curculionidae): Diversity, Density, and Associations With Host Reproduction
The Symbiotic Bacteria Nardonella in Rice Water Weevil (Coleoptera: Curculionidae): Diversity, Density, and Associations With Host Reproduction
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稻水象甲中的共生细菌纳尔多氏菌（鞘翅目：象甲科）：多样性、密度以及与宿主繁殖的关联
DOI：
10.1093/aesa/saw015
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发表时间：
2016-05-01
影响因子：
2.3
通讯作者：
Jiang, Mingxing
中科院分区：
文献类型：
--
作者：
Huang, Xu;Huang, Yunshan;Jiang, Mingxing
摘要 Nardonella（γ-proteobacteria）是一组古老的内共生细菌，仅存在于象鼻虫（Curculionoidea 超科）中。尽管在许多象鼻虫亚科中对它们的存在和系统发育进行了广泛的调查，但对它们的品系多样性、数量动态以及在宿主个体发育中的生物学作用知之甚少。在这里，基于对稻水象甲 Lissorhoptrus oryzophilus Kuschel（鞘翅目：象甲科）九个地理种群的细菌 16S RNA 基因序列的克隆和分析，我们鉴定了两个不同的纳尔多菌谱系。一种谱系分布广泛，存在于所调查的每个稻嗜乳杆菌种群中，而另一种谱系则在地理上受到很大限制，仅在一个种群中发现。这些细菌在象鼻虫的幼虫和蛹阶段都具有低密度；然而，随着成年人的出现，它们大幅增加。在雌性成虫中，它们主要存在于卵巢中；肠道也被感染，但密度很低。通过用各种抗生素（利福平、四环素和庆大霉素）治疗成年雌性，并观察其繁殖力、卵子活力以及卵巢中存在的纳多氏菌和其他两种细菌（沃尔巴克氏体和立克次氏体）的密度，我们发现尽管存在大量的纳多氏菌，雌性却无法产生可存活的卵子。这表明纳多氏菌可能在米乳杆菌的繁殖中没有发挥关键作用。这些结果有望扩展我们对纳多菌生物学及其与象鼻虫繁殖的关系的了解。
Abstract Nardonella (gamma-proteobacteria) are a group of ancient endosymbiotic bacteria that occur only in weevils (superfamily Curculionoidea). Despite the extensive surveys of their existence and phylogeny done in a number of weevil subfamilies, little is known about their strain diversity, quantitative dynamics, and biological roles in the ontogeny of hosts. Here, based on the cloning and analyses of bacterial 16S RNA gene sequences from nine geographic populations of rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), we identified two distinct Nardonella lineages. One lineage was widespread, occurring in each of the L. oryzophilus populations investigated, whereas the other was much geographically limited and found in only one population. These bacteria had a low density in both larval and pupal stages of the weevil; however, they increased substantially as adults emerged. In female adults, they were harbored dominantly in ovary; guts were also infected, but the density was very low. By treating adult females with various antibiotics (rifampicin, tetracycline, and gentamycin) and observing their fecundity, egg viability, as well as the density of Nardonella and the other two bacteria (Wolbachia and Rickettsia) present in the ovary, we found females did not produce viable eggs despite the presence of abundant Nardonella. This suggests that Nardonella might be not playing critical roles in the reproduction of L. oryzophilus. The results are expected to extend our knowledge of the biology of Nardonella and their associations with weevils' reproduction.