Immunologic heterogeneity of tumor cell subpopulations from a single mouse mammary tumor.
Immunologic heterogeneity of tumor cell subpopulations from a single mouse mammary tumor.
复制标题
来自单个小鼠乳腺肿瘤的肿瘤细胞亚群的免疫异质性。
DOI：
--
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
G. Heppner
中科院分区：
文献类型：
--
作者：
F. Miller;G. Heppner
从单个BALB/cfC3H乳腺腺癌中获得的5个亚群（66、67、68H、168和4.10LM）用于评估肿瘤内免疫异质性。分别免疫BALB/c和BALB/cfC3H小鼠，体外用铬释放和微细胞毒性试验检测免疫动物淋巴细胞（LNC）对各亚群的细胞介导免疫（CMI），体内用Winn法检测免疫动物淋巴细胞（LNC）对各亚群的免疫能力。不同亚群的免疫原性差异显著。交叉反应的模式表明至少涉及两个决定因素，其中一个可能是病毒抗原。病毒抗原在4个亚群（66,68h， 168和4.10LM）上表达。另一个决定因素在BALB/c和BALB/cfC3H小鼠中均具有免疫原性，并在4个亚群（66、67、168和4.10lm）中表达。因此，1个亚群（68H）只表达病毒抗原，1个亚群（67）只表达其他抗原，3个亚群（66、168和4.10LM）两者都表达。行列式的表达表现出定性和定量的变化。亚群诱导CMI的相对有效性和对lnc介导的杀伤的相对敏感性表明了数量上的差异。某些亚居对之间存在单向交叉反应，表明了质性差异；一个决定因素可以表达，使得亚群可以诱导细胞毒性细胞，但对它们不敏感，反之亦然。
Five subpopulations (66, 67, 68H, 168, and 4.10LM) obtained from a single BALB/cfC3H mammary adenocarcinoma were used to assess intratumor immunologic heterogeneity. BALB/c and BALB/cfC3H mice were immunized with each of the subpopulations, and lymph node cells (LNC) from immunized animals were tested for cell-mediated immunity (CMI) to each subpopulation in vitro by chromium release and microcytotoxicity tests and in vivo by Winn assays. The immunogenic character of the subpopulations differed markedly. The pattern of cross-reactivity indicated that at least two determinants were involved, one of which was probably a viral antigen. The viral antigen was expressed on 4 subpopulations (66, 68H, 168, and 4.10LM). The other determinant was immunogenic in both BALB/c and BALB/cfC3H mice and was expressed on 4 subpopulations (66, 67, 168, and 4.10lm). Thus 1 subpopulation (68H) expressed only the viral antigen, 1 (67) expressed only the other antigen, and 3 (66, 168, and 4.10LM) expressed both. Expression of the determinants showed qualitative and quantitative variations. Quantitative differences were noted by the relative effectiveness of the subpopulations to induce CMI and by the relative sensitivities to LNC-mediated killing. Qualitative differences were indicated by the occurrence of unidirectional cross-reactivities between some pairs of subpopulations; a determinant could be expressed so that the subpopulation could induce cytotoxic cells but not be sensitive to them or vice versa.