Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread
Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread
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DOI：
10.1073/pnas.1220272110
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发表时间：
2013-05-21
影响因子：
11.1
通讯作者：
Robey, Ellen A.
中科院分区：
文献类型：
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作者：
Coombes, Janine L.;Charsar, Brittany A.;Robey, Ellen A.
弓形虫感染通过口服途径发生，但我们缺乏关于寄生虫如何与肠道中宿主免疫系统相互作用的重要信息。我们使用双光子激光扫描显微镜结合小鼠口腔T。弓形虫感染来解决这个问题。T.弓形虫在小肠中建立了离散的感染灶，引发中性粒细胞和炎症单核细胞的募集和经上皮迁移。嗜中性粒细胞在主动侵入的细胞中占很大比例，我们提供的证据表明，嗜中性粒细胞和其他免疫细胞在T细胞扩散中的作用。通过肠腔感染弓形虫。我们的数据表明中性粒细胞是T。弓形虫感染，并提出了一个令人惊讶的逆行途径寄生虫传播的肠道。
Toxoplasma gondii infection occurs through the oral route, but we lack important information about how the parasite interacts with the host immune system in the intestine. We used two-photon laser-scanning microscopy in conjunction with a mouse model of oral T. gondii infection to address this issue. T. gondii established discrete foci of infection in the small intestine, eliciting the recruitment and transepithelial migration of neutrophils and inflammatory monocytes. Neutrophils accounted for a high proportion of actively invaded cells, and we provide evidence for a role for transmigrating neutrophils and other immune cells in the spread of T. gondii infection through the lumen of the intestine. Our data identify neutrophils as motile reservoirs of T. gondii infection and suggest a surprising retrograde pathway for parasite spread in the intestine.