Electrical impedance myography: Transitioning from human to animal studies
Electrical impedance myography: Transitioning from human to animal studies
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DOI：
10.1016/j.clinph.2006.03.024
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发表时间：
2006-08-01
影响因子：
4.7
通讯作者：
Rutkove, Seward B.
中科院分区：
文献类型：
--
作者：
Nie, Rui;Sunmonu, N. Abimbola;Rutkove, Seward B.
目的：探讨大鼠电阻抗肌图（EIM）的可行性。方法：对6只健康成年大鼠腘绳肌进行电刺激，施加频率为2 ~ 300 kHz。研究为期6周，其中3只大鼠进行皮肤记录（表面EIM）， 3只大鼠直接进行肌肉记录（直接肌肉EIM）。此外，我们对一只大鼠进行了坐骨神经压迫，并对损伤前后进行了比较。测量电抗和电阻，并计算主要结果变量相角(0)。结果：大鼠腘绳肌的EIM模式在质量上与人类受试者相似。这对表面和直接肌肉记录都成立，尽管直接肌肉数据的可重复性较低。在单个大鼠的坐骨神经挤压数据显示了相位的显著减少和频率依赖性的相对丧失。结论：可以从大鼠肌肉中获得与人类受试者相似的EIM数据，表面记录比直接肌肉记录更一致，更容易获得。坐骨神经压迫所见的变化与神经源性损伤患者的变化相一致。意义：这些结果支持在神经肌肉疾病大鼠模型上进行EIM的可能性。(c) 2006年国际临床神经生理学联合会。爱思唯尔爱尔兰有限公司出版。版权所有。
Objective: To determine the feasibility of performing electrical impedance myography (EIM) in rats.Methods: EIM was performed on the hamstring muscles of 6 healthy adult rats with applied frequencies of 2-300 kHz. Studies were performed over a 6-week period, with 3 rats having recordings made from the skin (surface EIM) and 3 with recordings directly from the muscle (direct-muscle EIM). In addition, sciatic nerve crush was performed on one rat and comparisons made pre- and post-injury. Reactance and resistance were measured and the primary outcome variable, the phase angle (0), calculated.Results: EIM patterns in the rat hamstring muscles were qualitatively similar to those observed in human subjects. This held true for both surface and direct-muscle recordings, although direct-muscle data appeared less repeatable. Sciatic nerve crush data in the single rat showed a dramatic reduction in phase and a relative loss of frequency-dependence.Conclusions: EIM data similar to that obtained from human subjects can be acquired from rat muscles with surface recordings proving more consistent and easier to obtain than direct-muscle recordings. Changes seen with sciatic nerve crush mirror those seen in patients with neurogenic injury.Significance: These results support the possibility of performing EIM on rat models of neuromuscular disease. (c) 2006 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.