Induction of secretory phospholipase A2 confirms the systemic inflammatory nature of adjuvant arthritis
Induction of secretory phospholipase A2 confirms the systemic inflammatory nature of adjuvant arthritis
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DOI：
10.1023/a:1022336006109
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发表时间：
1998-04-01
期刊：
影响因子：
5.1
通讯作者：
Pruzanski, W
中科院分区：
文献类型：
--
作者：
Lin, MKS;Katz, A;Pruzanski, W
佐剂性关节炎（AA）是一种公认的炎症性关节炎模型。然而，到目前为止，很少有关于炎症介质的信息，特别是在AA中花生四烯酸级联反应。我们的目的是研究分泌型（sPLA（2））和胞质型（cPLA（2））磷脂酶A（2）在AA过程中的不同器官的表达。在第0、7、14、21、28和42天处死的刘易斯大鼠中诱导AA。检测大鼠足爪、区域淋巴结、脾、肝、肺和主动脉中sPLA（2）mRNA、蛋白和cPLA（2）mRNA的表达。血清sPLA（2）活性在第21天从15213 +/- 1131增加到最大值32455 +/- 4109 nmol/30'。在第14天观察到爪、肺和主动脉中sPLA（2）mRNA的最大增加，在第28天观察到淋巴结和脾脏中sPLA（2）mRNA的最大增加。在肝脏中，发现痕量水平，没有相应的蛋白质表达。在爪、肺、主动脉和淋巴结中，在第14天观察到sPLA（2）蛋白的最大增加，而脾脏在AA期间显示恒定的sPLA（2）蛋白水平。cPLA（2）mRNA在AA的所有器官中均检测到，在AA过程中没有显著变化，但区域淋巴结除外，其信息在14至28天之间增加。sPLA（2）的mRNA和蛋白在几个器官中的诱导是AA是全身性炎症过程的证据。sPLA（2）的表达与炎症反应的严重程度平行，提示sPLA（2）可能在AA中起致病作用。cPLA（2）mRNA缺乏增强可能意味着这种酶在AA中不被诱导，或者在炎症过程的早期增加。
Adjuvant arthritis (AA) is an accepted model of inflammatory arthritis. Until now, however, there is little information about inflammatory mediators, specifically in relation to the arachidonic acid cascade in AA. Our objective was to study the expression of secretory (sPLA(2)) and cytosolic (cPLA(2)) phospholipases A(2) in various organs during the course of AA. AA was induced in Lewis rats which were sacrificed at days 0, 7, 14, 21, 28 and 42. Expression of sPLA(2) mRNA and protein and mRNA of cPLA(2) in paws, regional lymph nodes, spleen, liver lungs and aorta was investigated. Serum sPLA(2) activity increased from 15213 +/- 1131 to a maximum of 32455 +/- 4109 nmol/30' on day 21. Maximal increase in sPLA(2) mRNA in paws, lung and aorta was observed on day 14, and in the lymph nodes and spleen on day 28. In the liver, trace levels were found with no corresponding protein expression. In paws, lung, aorta and lymph nodes maximum increase in sPLA(2) protein was noted on day 14 whereas the spleen showed constant sPLA(2) protein level during AA. cPLA(2) mRNA detected in all organs, did not significantly change during the course of AA, with the exception of regional lymph nodes where the message increased between 14 and 28 day. Induction of mRNA and protein of sPLA(2) in several organs is an evidence that AA is a systemic inflammatory process. The parallelity of the sPLA(2) expression to the severity of inflammatory process, implies that sPLA(2) may play pathogenic role in AA. Lack of enhancement of cPLA(2) mRNA may mean that this enzyme is either not induced in AA, or it increases earlier in the course of the inflammatory process.