G and P genotypes of group A rotavirus from diarrhoeic calves born to cows vaccinated against the NCDV (P[1],G6) rotavirus strain
G and P genotypes of group A rotavirus from diarrhoeic calves born to cows vaccinated against the NCDV (P[1],G6) rotavirus strain
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DOI：
10.1111/j.1439-0450.2004.00737.x
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发表时间：
2004-04-01
期刊：
影响因子：
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通讯作者：
Alfieri, AA
中科院分区：
文献类型：
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作者：
Barreiros, MAB;Alfieri, AF;Alfieri, AA
应用多重套式逆转录聚合酶链式反应(RT-PCR)对71株A组轮状病毒野毒株进行基因分型。2000年1月至2001年12月期间，从巴西南马托格罗索州、圣保罗和巴拉那州的14个奶牛群和肉牛群中采集了腹泻小牛的粪便样本。从7个鸡群接种P[1]、G6株轮状病毒商业灭活疫苗的母牛粪便中检出35株轮状病毒野毒株，从其他7个未接种轮状病毒的母牛腹泻犊牛粪便中检出36株轮状病毒野毒株。94.3%(67/71)的菌株可鉴定出P、G轮状病毒基因型别。但有两株未检出G基因，另一株未检出P基因，另一株未检出这两种基因。未接种轮状病毒和接种轮状病毒的轮状病毒野毒株中，P和G轮状病毒基因关联率分别为94.4%(34/36)和94.3%(33/35)。在非免疫猪群中，二元组合为P[L]、G6(三群，12株)、P[1]、G8(三群，六株)、P[5]，G6(两群，10株)和P[L]，G8(一群，四株)。免疫猪群中P[L 1]、G6等位基因频率最高(3群，12株)，其次是P[5]，G6(2群，3株)，P[11]，GS(2群，8株)。在1个未接种疫苗的牛群(2株)和4个接种疫苗的牛群(10株)中观察到混合感染。在来自未接种疫苗的牛群的一个菌株和来自接种疫苗的牛群的两个菌株中，无法鉴定二元组合。在未接种疫苗的牛群中，异常基因型别的出现率(91.4%)高于牛轮状病毒株的原型。在从接种疫苗的母牛中出生的腹泻小牛中检测到的轮状病毒现场毒株中，没有发现内布拉斯加州小牛腹泻病毒(NCDV)样基因。
A total of 71 group A rotavirus field strains, detected by polyacrylamide gel electrophoresis and enzyme immune assay, were submitted to genotyping by a multiplex nested-reverse transcription-polymerase chain reaction (RT-PCR). Faecal samples were obtained from calves with diarrhoea, between January 2000 and December 2001, from 14 dairy and beef cattle herds located in Mato Grosso do Sul, Sao Paulo and Parana States, Brazil. Thirty-five rotavirus field strains were detected in faeces from calves born to vaccinated dams with inactivated commercial vaccine containing the rotavirus Strain P[1],G6 from seven herds while 36 rotavirus field strains were obtained from diarrhoeic calves from other seven herds without rotavirus vaccination. Identification of P and G rotavirus genotypes was possible in 94.3% (67/71) of the strains. However, G genotype could not be identified in two strains, P genotype in another strain and both genotypes were not detected in a third strain. P and G rotavirus genotype associations were identified in rotavirus field strains from non-vaccinated and vaccinated herds at similar rates of 94.4% (34/36) and 94.3% (33/35), respectively. In non-vaccinated herds binary combinations were P[l],G6 (three herds, 12 strains), P[1],G8 (three herds; six strains), P[5],G6 (two herds; 10 strains) and P[l],G8 (one herd; four strains). The genotype P[l 1],G6 was the most frequent (three herds; 12 strains) in vaccinated herds, followed by P[5],G6 (two herds; three strains), and P[11],GS (two herds; eight strains). Mixed infections were observed in one non-vaccinated herd (two strains) and in four vaccinated herds (10 strains). Identification of binary combinations was not possible in one strain from non-vaccinated herds and two strains from vaccinated herds. Unusual genotypes were more frequent (91.4%) in non-vaccinated herds than those from prototypes of bovine rotavirus strains. Nebraska calf diarrhoea virus (NCDV)-like genotype was found in none of the rotavirus field strains detected in diarrhoeic calves born of vaccinated dams.