A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery.
A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery.
复制标题
一种新型生物传感器，用于监测渗透胁迫和恢复下豌豆根分泌物和根瘤中的脯氨酸。
DOI：
10.1007/s11104-020-04577-2
复制
发表时间：
2020
期刊：
影响因子：
4.9
通讯作者：
Rubia MI
中科院分区：
文献类型：
--
作者：
Rubia MI
Background and aimsPlant and bacteria are able to synthesise proline, which acts as a compound to counteract the negative effects of osmotic stresses. Most methodologies rely on the extraction of compounds using destructive methods. This work describes a new proline biosensor that allows the monitoring of proline levels in a non-invasive manner in root exudates and nodules of legume plants.MethodsThe proline biosensor was constructed by cloning the promoter region of pRL120553, a gene with high levels of induction in the presence of proline, in front of theluxcassette inRhizobium leguminosarumbv.viciae.ResultsFree-living assays show that the proline biosensor is sensitive and specific for proline. Proline was detected in both root exudates and nodules of pea plants. The luminescence detected in bacteroids did not show variations during osmotic stress treatments, but significantly increased during recovery.ConclusionsThis biosensor is a useful tool for thein vivomonitoring of proline levels in root exudates and bacteroids of symbiotic root nodules, and it contributes to our understanding of the metabolic exchange occurring in nodules under abiotic stress conditions.
