Phenotyping and Genotype x Environment Interaction of Resistance to Leaffolder, Cnaphalocrocis medinalis Guenee (Lepidoptera: Pyralidae) in Rice
Phenotyping and Genotype x Environment Interaction of Resistance to Leaffolder, Cnaphalocrocis medinalis Guenee (Lepidoptera: Pyralidae) in Rice
复制标题
DOI：
10.3389/fpls.2019.00049
复制
发表时间：
2019-02-18
影响因子：
5.6
通讯作者：
Katti, Gururaj
中科院分区：
文献类型：
--
作者：
Chintalapati, Padmavathi;Balakrishnan, Divya;Katti, Gururaj
稻纵卷叶螟（Cnaphalocrocis medinalis）是亚洲主要的食叶昆虫之一，因其造成严重的产量损失而备受关注。破坏的高能见度促使农民使用有毒农药进行管理。因此，寻找新的稳定的叶黄菌抗性来源至关重要。对抗性亲本W1263与易感亲本TN1杂交的160个重组自交系（RILs）进行了3个季节的快速田间筛选，鉴定出9个RILs为水稻叶枯病抗性的稳定来源。表型频率分布连续，表明该抗性是一种由多基因控制的数量性状。采用基因型和基因型x环境相互作用（GGE）分析方法对3个季节的表型数据进行分析，以鉴定稳定抗性品系。加性主效应和乘法互作（AMMI）分析表明，基因型（GEN）效应占受损叶面积总平方的86.41%；对环境（ENV）效应的影响为0.48%，对环境（G × E）互作效应的影响为5.68%。受害面积、受害分数和叶长在三种环境中表现出非常高的广义遗传力。但叶宽遗传力较低，表明环境影响较大。系统发育分析根据耐药反应将160个抗性基因及其亲本分成5类。AMMI和GGE双图分析表明，危害面积和危害评分较低的稳定基因型G8 （MP114）和G3 （MP108）可用于培育抗叶病品种。
Rice leaffolder, Cnaphalocrocis medinalis is one of the key foliage feeding insects of great concern throughout Asia as it results in significant yield losses. High visibility of damage is triggering farmers to apply toxic pesticides for its management. Therefore, it is vital to identify new stable sources of resistance for leaffolder. Phenotyping of 160 recombinant inbred lines (RILs) of a cross between a resistant parent, W1263 and a susceptible parent, TN1 using a rapid field screening method for three seasons resulted in identification of nine RILs as stable sources of resistance to rice leaffolder. Phenotypic frequency distributions were found continuous indicating that the resistance is a quantitative trait governed by polygenes. Phenotypic data for three seasons were analyzed using Genotype and Genotype x Environment Interaction (GGE) analysis for identification of stable resistant lines. Additive main effect and multiplicative interaction (AMMI) analysis showed that 86.41% of the total sum of square of damaged leaf area was attributed to genotype (GEN) effect; 0.48% to environment (ENV) effects and 5.68% to genotype by environment (G x E) interaction effects. Damage area, damage score and leaf length showed very high broad-sense heritability across three environments. However, leaf width had low heritability indicating higher environment influence. Phylogenetic analysis grouped these 160 RILs and parents into five clusters based on resistant reaction. AMMI and GGE biplot analysis revealed that stable genotypes G8 (MP114) and G3 (MP108) with lower damage area and damage score can be utilized in developing cultivars with leaffolder resistance.