Serological survey of SARS-CoV-2 for experimental, domestic, companion and wild animals excludes intermediate hosts of 35 different species of animals
Serological survey of SARS-CoV-2 for experimental, domestic, companion and wild animals excludes intermediate hosts of 35 different species of animals
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DOI：
10.1111/tbed.13577
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发表时间：
2020-05-07
影响因子：
4.3
通讯作者：
Tian, Kegong
中科院分区：
文献类型：
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作者：
Deng, Junhua;Jin, Yipeng;Tian, Kegong
SARS冠状病毒2型（SARS-CoV-2）已在123个国家发生大流行，死亡人数超过5,000人，但病毒的原始宿主和中间宿主仍不清楚。本研究采用双抗原夹心ELISA法对35种动物共1,914份血清标本进行了SARS-CoV-2特异性抗体的检测，并对其特异性和敏感性进行了验证。结果表明，上述标本均未检测到SARS-CoV-2特异性抗体，排除了35种动物为SARS-CoV-2中间宿主的可能性。更重要的是，宠物狗（包括SARS-CoV-2患者饲养的一只狗和与其有密切接触的两只狗）和猫、流浪狗和猫等伴侣动物也显示SARS-CoV-2血清学阴性，这缓解了公众对宠物作为SARS-CoV-2携带者的担忧。
The pandemic SARS-CoV-2 has been reported in 123 countries with more than 5,000 patients died from it. However, the original and intermediate hosts of the virus remain unknown. In this study, 1,914 serum samples from 35 animal species were used for detection of SARS-CoV-2-specific antibodies using double-antigen sandwich ELISA after validating its specificity and sensitivity. The results showed that no SARS-CoV-2-specific antibodies were detected in above samples which excluded the possibility of 35 animal species as intermediate host for SARS-CoV-2. More importantly, companion animals including pet dogs (including one dog the SARS-CoV-2 patient kept and two dogs which had close contact with it) and cats, street dogs and cats also showed serological negative to SARS-CoV-2, which relieved the public concerns for the pets as SARS-CoV-2 carriers.