SINE-Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation.
SINE-Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation.
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DOI：
10.1002/advs.202307505
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发表时间：
2024-01
期刊：
影响因子：
15.1
通讯作者：
Jin, Jun-Xue
中科院分区：
文献类型：
--
作者：
He, Tianyao;Peng, Jinyu;Yang, Shu;Liu, Dongsong;Gao, Shuang;Zhu, Yanlong;Chai, Zhuang;Lee, Byeong Chun;Wei, Renyue;Wang, Jiaqiang;Liu, Zhonghua;Jin, Jun-Xue
在小鼠中，反转录转座子相关的长非编码RNA(LncRNA)在着床前发育中发挥着重要的调节作用；然而，它们是否在猪着床前发育中发挥作用在很大程度上还不清楚。本研究旨在筛选猪早期胚胎中与反转录转座子相关的LncRNA，并鉴定一种猪8细胞胚胎特异的与Sine相关的核长非编码RNA SAWPA。SAWPA对猪胚胎发育是必不可少的，因为SAWPA的缺失导致8-细胞期的发育停滞，并伴随着JNK-MAPK信号通路的抑制。从机制上讲，SAWPA作为JNK的反式转录因子，与HNRNPA1和MED8形成RNA-蛋白质复合体，与JNK上游的正弦元件结合。因此，SAWPA作为猪体内第一个具有功能的与正弦相关的长非编码RNA，为哺乳动物着床前发育中反转录转座子的机制研究提供了新的思路。在猪早期胚胎发育过程中，Sine相关的长非编码RNA SAWPA与MED8和HNRNPA1结合，通过Sine序列调节JNK的转录，影响合子基因组的激活。SAWPA的缺失会影响JNK-MAPK信号通路，导致胚胎发育受阻于8-细胞期。
In mice, retrotransposon‐associated long noncoding RNAs (lncRNA) play important regulatory roles in pre‐implantation development; however, it is largely unknown whether they function in the pre‐implantation development in pigs. The current study aims to screen for retrotransposon‐associated lncRNA in porcine early embryos and identifies a porcine 8‐cell embryo‐specific SINE‐associated nuclear long noncoding RNA named SAWPA. SAWPA is essential for porcine embryonic development as depletion of SAWPA results in a developmental arrest at the 8‐cell stage, accompanied by the inhibition of the JNK‐MAPK signaling pathway. Mechanistically, SAWPA works in trans as a transcription factor for JNK through the formation of an RNA‐protein complex with HNRNPA1 and MED8 binding the SINE elements upstream of JNK. Therefore, as the first functional SINE‐associated long noncoding RNAs in pigs, SAWPA provides novel insights for the mechanism research on retrotransposons in mammalian pre‐implantation development. During the early embryonic development of pigs, the SINE‐associated long non‐coding RNA SAWPA binds to MED8 and HNRNPA1 to regulate the transcription of JNK through SINE sequences, affecting the activation of the zygotic genome. Depletion of SAWPA leads to an impact on the JNK‐MAPK signaling pathway, causing embryonic development to become arrested at the 8‐cell stage.
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影响因子：
5.4
作者：
Bruun GH;Doktor TK;Borch-Jensen J;Masuda A;Krainer AR;Ohno K;Andresen BS
通讯作者：
Andresen BS
DOI：
10.1242/dev.116996
发表时间：
2015-03-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
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通讯作者：
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影响因子：
3.8
作者：
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通讯作者：
Duranthon, V.
影响因子：
64.8
作者：
BRAUDE, P;BOLTON, V;MOORE, S
通讯作者：
MOORE, S
发现一种新型长非编码 RNA Lx8-SINE B2 作为多能性标记
影响因子：
4.3
作者：
Chen F;Zhang M;Feng X;Li X;Sun H;Lu X
通讯作者：
Lu X