Cloning and expression of the human p8, a nuclear protein with mitogenic activity.
Cloning and expression of the human p8, a nuclear protein with mitogenic activity.
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DOI：
10.1046/j.1432-1327.1999.00092.x
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发表时间：
2001-12
期刊：
影响因子：
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通讯作者：
S. Vasseur;G. Mallo;F. Fiedler;H. Bödeker;E. Cánepa;S. Moreno;J. Iovanna
中科院分区：
文献类型：
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作者：
S. Vasseur;G. Mallo;F. Fiedler;H. Bödeker;E. Cánepa;S. Moreno;J. Iovanna
我们之前已经发现了一种新的大鼠mRNA，暂时命名为p8，它在胰腺对急性胰腺炎的反应中表现出强烈但短暂的诱导作用。我们在这里报道了人类p8 cDNA的克隆和测序。人类的p8多肽有82个氨基酸长，与大鼠的p8多肽有74%的一致性。建立了p8基因的完整结构。p8基因由三个外显子组成，由两个内含子隔开，该基因被定位在第16号染色体上。对p8初级结构的分析表明存在核靶向的二部基序，表明p8可能在细胞核内起作用。用p8 cDNA转染COS-7细胞，然后用特异性抗体进行免疫染色，证实了这一假设。血清饥饿和神经酰胺可诱导部分（但不是全部）细胞表达p8 mRNA。为了分析p8的功能，我们用p8表达质粒稳定转染HeLa细胞，观察其生长情况和对凋亡的敏感性。对TNF α和staurosporine诱导的细胞凋亡的反应不受p8表达的影响。然而，表达p8的细胞生长明显更快。
We have previously identified a new rat mRNA, provisionally named p8, which showed a strong, but transient, induction in the pancreas in response to acute pancreatitis. We report here the cloning and sequencing of the human p8 cDNA. The human p8 polypeptide is 82 amino acids long and shows an overall identity of 74% with the rat counterpart. The complete structure of the p8 gene was also established. The p8 gene comprises three exons separated by two introns and the gene was mapped to chromosome 16. Analysis of the p8 primary structure suggested the presence of a bipartite motif of nuclear targeting, indicating that p8 may function within the nucleus. This presumption has been confirmed by transfection of COS-7 cells with the p8 cDNA followed by immunostaining with specific antibodies. p8 mRNA expression is induced in some, but not all, cells by serum starvation and ceramide. To analyze the p8 function, we stably transfected HeLa cells with a p8 expression plasmid, and measured their growth and their sensitivity to apoptosis. Response to TNF alpha and staurosporine-induced apoptosis was not modified by p8 expression. However, cells expressing p8 grew significantly more rapidly.