Polyhydroxyalkanoate biosynthesis in Pseudomonas pseudoalcaligenes YS1
Polyhydroxyalkanoate biosynthesis in Pseudomonas pseudoalcaligenes YS1
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DOI：
10.1111/j.1574-6968.2002.tb11247.x
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发表时间：
2002-06-18
影响因子：
2.1
通讯作者：
Chen, GQ
中科院分区：
文献类型：
--
作者：
Hang, XM;Lin, ZX;Chen, GQ
Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (fadB::Tet) was constructed to express the cloned PHA synthesis gene phaC1(Pp) or phaC2(Pp). The fadB deleted mutant KM32B harboring phaC1(Pp) or phaC2(Pp) showed mcl PHA accumulation while the intact E coli KM32 did not. The results demonstrated that P. pseudoalcaligenes YS1 possessed at least two PHA synthesis pathways; one of them was responsible for production of mcl PHA. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
