RAT ANTI-T15 MONOCLONAL-ANTIBODIES WITH SPECIFICITY FOR VH-EPITOPES AND VH-VL-EPITOPES
RAT ANTI-T15 MONOCLONAL-ANTIBODIES WITH SPECIFICITY FOR VH-EPITOPES AND VH-VL-EPITOPES
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DOI：
10.1016/0161-5890(84)90154-8
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发表时间：
1984-01-01
影响因子：
3.6
通讯作者：
SCHARFF, MD
中科院分区：
文献类型：
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作者：
DESAYMARD, C;GIUSTI, AM;SCHARFF, MD
用磷酸胆碱（PC）免疫的 BALB/c 小鼠产生的抗体主要是 T15 独特型。许多实验室通过同种异体或同基因免疫产生单克隆抗 T15 抗体。大多数这些小鼠单克隆抗体与 PC 结合位点内或附近的独特位发生反应，并且需要 T15 H 和 T15 L 链可变区的存在。通过用 T15 Ig 免疫大鼠，获得了识别不靠近抗原结合位点的独特位点的单克隆抗体。其中四种大鼠抗 T15 单克隆抗体与游离 T15 H 链以及与不相关 L 链相关的 T15 H 链反应，而其他 4 种大鼠单克隆抗体需要 T15 H 和 T15 L 链的存在。这组大鼠抗 T15 单克隆抗体可用于寻找 T15 抗体内的异质性以及跟踪不同品系小鼠中 T15 H 链可变区的表达。
BALB/c mice immunized with phosphorylcholine (PC) produce antibodies which are predominantly of the T15 idiotype. Monoclonal anti-T15 antibodies were generated in a number of laboratories by allogenic or syngenic immunization. Most of these mouse monoclonal antibodies react with idiotopes that are in or near the PC-binding site and require the presence of both T15 H and T15 L chain variable regions. By immunizing rats with T15 Ig, monoclonal antibodies were obtained that recognize idiotopes that are not near the antigen-binding site. Four of these rat anti-T15 monoclonal antibodies react with free T15 H chains and with T15 H chains associated with irrelevant L chains while 4 other rat monoclonal antibodies require the presence of both T15 H and T15 L chains. This battery of rat anti-T15 monoclonal antibodies is useful in searching for heterogeneity within the T15 antibodies and in following the expression of the T15 H chain variable region in different strains of mice.