Microfluidic-SERS devices for one shot limit-of-detection.

Microfluidic-SERS devices for one shot limit-of-detection.
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DOI:
10.1039/c4an00357h
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发表时间:
2014-07-07
期刊:
The Analyst
影响因子:
--
通讯作者:
Haynes CL
Haynes CL
中科院分区:
其他
文献类型:
--
作者:
Kim D;Campos AR;Datt A;Gao Z;Rycenga M;Burrows ND;Greeneltch NG;Mirkin CA;Murphy CJ;Van Duyne RP;Haynes CL

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微流体传感平台利用各种光学信号转导机制促进并行、低样本量检测。在这里,我们介绍了一种简单的混合微流体装置,能够对引入的分析物溶液进行连续稀释,并终止于五个离散的传感元件。我们展示了该设备在芯片上荧光和表面增强拉曼散射 (SERS) 分析物检测方面的实用性,并且我们演示了设备与传统的非柔性 SERS 基底和直接纳入微流体通道中的 SERS 活性纳米粒子结合使用以创建灵活的 SERS 平台。结果表明,在不同的灵敏度下,灵活或非灵活的设​​备都可以轻松地用于创建校准曲线并通过单个实验进行检测限研究。
Microfluidic sensing platforms facilitate parallel, low sample volume detection using various optical signal transduction mechanisms. Herein, we introduce a simple mixing microfluidic device, enabling serial dilution of introduced analyte solution that terminates in five discrete sensing elements. We demonstrate the utility of this device with on-chip fluorescence and surface-enhanced Raman scattering (SERS) detection of analytes, and we demonstrate device use both when combined with a traditional inflexible SERS substrate and with SERS-active nanoparticles that are directly incorporated into microfluidic channels to create a flexible SERS platform. The results indicate, with varying sensitivities, that either flexible or inflexible devices can be easily used to create a calibration curve and perform a limit of detection study with a single experiment.
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发表时间: 2003-12-03
影响因子: 15
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