Piwi-interacting RNA (piRNA) expression patterns in pearl oyster (Pinctada fucata) somatic tissues
Piwi-interacting RNA (piRNA) expression patterns in pearl oyster (Pinctada fucata) somatic tissues
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DOI：
10.1038/s41598-018-36726-0
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发表时间：
2019-01-22
影响因子：
4.6
通讯作者：
Asakawa, Shuichi
中科院分区：
文献类型：
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作者：
Huang, Songqian;Ichikawa, Yuki;Asakawa, Shuichi
Piwi相互作用RNA（piRNA）属于最近发现的一类小的非编码RNA，其最好理解的功能是抑制转座因子活性。大多数皮尔纳研究都是在模式生物上进行的，对软体动物中皮尔纳的表达和功能知之甚少。我们对合浦珠母贝的外套膜、闭壳肌、鳃和卵巢组织中提取的小RNA进行了高通量测序。序列约为30 nt的RNA种类在所有组织中广泛表达。5'末端的尿苷和3'末端的β-消除保护表明这些是推定的piRNA。总共1800万个推定的piRNA被分配给280万个独特的piRNA，并且鉴定了35,848个皮尔纳簇。映射到参考基因组显示，25%的独特piRNA映射到支架上的多个串联基因座。皮尔纳簇的表达模式在体细胞组织内相似，但在体细胞和性腺组织之间显著不同。这些发现表明，在珍珠牡蛎中，piRNA具有超越生殖系的重要和新颖的功能。
Piwi-interacting RNAs (piRNAs) belong to a recently discovered class of small non-coding RNAs whose best-understood function is repressing transposable element activity. Most piRNA studies have been conducted on model organisms and little is known about piRNA expression and function in mollusks. We performed high-throughput sequencing of small RNAs extracted from the mantle, adductor muscle, gill, and ovary tissues of the pearl oyster, Pinctada fucata. RNA species with sequences of approximately 30 nt were widely expressed in all tissues. Uridine at the 5' terminal and protection from beta-elimination at the 3' terminal suggested that these were putative piRNAs. A total of 18.0 million putative piRNAs were assigned to 2.8 million unique piRNAs, and 35,848 piRNA clusters were identified. Mapping to the reference genome showed that 25% of the unique piRNAs mapped to multiple tandem loci on the scaffold. Expression patterns of the piRNA clusters were similar within the somatic tissues, but differed significantly between the somatic and gonadal tissues. These findings suggest that in pearl oysters piRNAs have important and novel functions beyond those in the germ line.