Origin of the epaxial and hypaxial myotome in avian embryos
Origin of the epaxial and hypaxial myotome in avian embryos
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DOI：
10.1007/s004290000130
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发表时间：
2000-11-01
期刊：
影响因子：
--
通讯作者：
Christ, B
中科院分区：
文献类型：
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作者：
Huang, RJ;Christ, B
肌节起源于皮肌节。关于肌节前体细胞在皮肌节内的位置以及它们与皮肌节的分离存在争议。在这里，我们解决了问题的肌节形成通过标记皮肤肌节细胞使用鹌鹑-小鸡标记技术。我们进行了五个系列的移植：(1)内侧1/3，(2)中间1/3，(3)外1/3，(4)颅侧半，(5)胸段皮肌刀的尾侧半侧。嫁接程序是在HH阶段的15-17期鹌鹑和雏鸡胚胎上进行的。将嵌合体重新孵化2天，直到HH阶段24-25。所有的移植部分都形成了肌小刀。上轴肌节起源于皮肌节的内侧1/3，而下轴肌节由皮肌节的中间和外侧的1/3形成。EP和下轴肌节结构域在肌节最厚的部位相遇，位于肌节腹外侧轴的中间。肌节在上轴域的生长早于下轴域。皮肌节的头缘和尾缘对上轴和下轴肌瘤的作用是一样的。第一批出生的肌节细胞位于外轴肌节的外侧部分，然后在内侧和外侧进行发育。
The myotome originates from the dermomyotome. Controversy surrounds the location of myotome precursor cells within the dermomyotome and their segregation from the dermomyotome. Here we addressed the problem of myotome formation by labeling dermomyotome cells using the quail-chick marking technique. We carried out five series of transplantation and replaced: (1) the medial third, (2) the intermediate third, (3) the lateral third, (4) the cranial half, (5) the caudal half of a thoracic dermomyotome. The grafting procedures were performed in HH-stages 15-17 of quail and chick embryos. The chimeras were reincubated for 2 days up to HH-stages 24-25. All of the grafted parts contributed to the myotome. The epaxial myotome is derived from the medial third of the dermomyotome, while the hypaxial myotome is formed by both the intermediate and lateral third of the dermomyotome. Ep- and hypaxial myotome domains meet in the thickest part of the myotome that is situated in the middle of its ventrolateral axis. Myotome growth in the epaxial domain begins earlier than in the hypaxial domain. Cranial and caudal edges of the dermomyotome contribute equally to both the epaxial and hypaxial myotomes. The first born myotome cells are located in the lateral part of the epaxial myotome and development then proceedes in medial and lateral directions.