GOLF - AN OLFACTORY NEURON SPECIFIC G-PROTEIN INVOLVED IN ODORANT SIGNAL TRANSDUCTION
GOLF - AN OLFACTORY NEURON SPECIFIC G-PROTEIN INVOLVED IN ODORANT SIGNAL TRANSDUCTION
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DOI：
10.1126/science.2499043
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发表时间：
1989-05-19
期刊：
影响因子：
56.9
通讯作者：
REED, RR
中科院分区：
文献类型：
--
作者：
JONES, DT;REED, RR
生化和电生理研究表明，气味通过G蛋白介导的腺苷酸环化酶级联反应诱导嗅觉感觉神经元的反应。一种嗅觉特异性鸟苷三磷酸（GTP）结合蛋白。亚基现在已经被表征，证据表明，这种G蛋白，称为高尔夫，调解嗅觉。信使RNA编码golf。alpha。在嗅觉神经上皮细胞中表达，但在其他六种组织中不表达。此外，在嗅上皮内，golf . α。似乎只由感觉神经元表达。特异抗血清用于定位golf α。受体神经元的感觉器官的蛋白质。Golf.alpha。与刺激G蛋白gs . α具有广泛的氨基酸同源性（88%）。golf。alpha的表达式。在S49 cyc- kin细胞中，缺乏内源性刺激G蛋白的细胞系显示其在异种系统中刺激腺苷酸环化酶的能力。
Biochemical and electrophysiological studies suggest that odorants induce responses in olfactory sensory neurons via an adenylate cyclase cascade mediated by a G protein. An olfactory-specific guanosine triphosphate (GTP)-binding protein .alpha. subunit has now been characterized and evidence is presented suggesting that this G protein, termed Golf, mediates olfaction. Messenger RNA that encodes Golf.alpha. is expressed in olfactory neuroephithelium but not in six other tissues tested. Moreover, within the olfactory epithelium, Golf.alpha. appears to be expressed only by the sensory neurons. Specific antisera were used to localize Golf.alpha. protein to the sensory apparatus of the receptor neurons. Golf.alpha. shares extensive amino acid identity (88 percent) with the stimulatory G protein, Gs.alpha.. The expression of Golf.alpha. in S49 cyc- kin- cells, a line deficient in endogenous stimulatory G proteins, demonstrates its capacity to stimulate adenylate cyclase in a heterologous system.