Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level.
Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level.
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DOI：
10.1364/boe.2.001803
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发表时间：
2011-07-01
影响因子：
3.4
通讯作者：
Myllylä R
中科院分区：
文献类型：
--
作者：
Kinnunen M;Kauppila A;Karmenyan A;Myllylä R
我们演示了使用双光束光镊系统，以稳定红细胞（RBC）的取向在光镊测量过程中的弹性光散射从被困细胞在5-30度的角度范围内。另一种激光（He-Ne）用于照射细胞，用测角仪和光电倍增管测量来自单个细胞的弹性光散射分布。此外，CCD相机图像的红细胞与激光照射和不作为补充信息。在不同的固定方向上测量来自RBC的光散射。当细胞的长度在两个不同的方向上改变时，也测量来自细胞的光散射。从球形和圆齿红细胞的光散射测量进行了描述，并与其他细胞取向的结果进行了比较。分析表明，测量的弹性光散射分布揭示了红细胞的取向和形状的变化。拉伸对散射变化的影响在He-Ne激光正面入射的情况下比在边缘入射的情况下更大。从不同方向的红细胞，以及从一个球形红细胞的散射模式进行了比较与文献中发现的数值结果。发现了良好的相关性。
We demonstrate the use of a double-beam optical tweezers system to stabilize red blood cell (RBC) orientation in the optical tweezers during measurements of elastic light scattering from the trapped cells in an angle range of 5-30 degrees. Another laser (He-Ne) was used to illuminate the cell and elastic light scattering distribution from the single cell was measured with a goniometer and a photomultiplier tube. Moreover, CCD camera images of RBCs with and without laser illumination are presented as complementary information. Light scattering from a RBC was measured in different fixed orientations. Light scattering from cells was also measured when the length of the cell was changed in two different orientations. Light scattering measurements from spherical and crenate RBCs are described and the results are compared with other cell orientations. Analysis shows that the measured elastic light scattering distributions reveal changes in the RBC’s orientation and shape. The effect of stretching on the changes in scattering is larger in the case of face-on incidence of He-Ne laser light than in rim-on incidence. The scattering patterns from RBCs in different orientations as well as from a spherical RBC were compared with numerical results found in literature. Good correlation was found.