Time Dependent Effects of 6-OHDA Lesions on Iron Level and Neuronal Loss in Rat Nigrostriatal System
Time Dependent Effects of 6-OHDA Lesions on Iron Level and Neuronal Loss in Rat Nigrostriatal System
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DOI：
10.1007/s11064-004-7031-5
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发表时间：
2004-12
影响因子：
4.4
通讯作者：
Jun Wang;Hong Jiang;Junxia Xie
中科院分区：
文献类型：
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作者：
Jun Wang;Hong Jiang;Junxia Xie
用6-羟基多巴胺(6-OHDA)单侧损毁大鼠，观察黑质纹状体系统铁含量的早期变化和神经元的丢失。结果表明：损伤后1、3、5、7、14天，黑质(SN)酪氨酸羟化酶免疫反应阳性细胞密度逐渐降低。6-OHDA损毁后1~14天，损毁的SN中铁水平升高，但三者之间铁水平无差异。除损伤后第14天损毁侧纹状体(Str)DA释放量减少外，其余各组均无变化。这些结果表明，黑质中铁水平的升高发生在DA神经元中度减少的时候。然而，由于DA系统的巨大代偿机制，STR的DA释放没有变化，直到TH-IR细胞高度减少。
The early changes in iron level and neuronal loss in rat nigrostriatal system were investigated using 6-hydroxydopamine (6-OHDA) unilaterally lesioned rats. The results showed that: 1, 3, 5, 7, and 14 days of postlesion, there was a progressive reduction in the density of the tyrosine hydroxylase immunoreactive (TH-ir) cells in the lesioned substantia nigra (SN). Iron level increased in the lesioned SN from 1–14 days following 6-OHDA lesions, but there were no differences in iron level among them. Only on 14 days of postlesion, did the DA release decrease in striatum (Str) of the lesioned side, while there were no changes in other groups. These results implied that the increased iron level in SN occured when there was a moderate reduction of DA neurons. However, the DA release in Str was unchanged until TH-ir cells were highly reduced due to the immense compensatory mechanism of the DA system.