Sigma A recognition sites in the Bacillus subtilis genome
Sigma A recognition sites in the Bacillus subtilis genome
复制标题
DOI：
10.1099/00221287-147-9-2417
复制
发表时间：
2001-09-01
期刊：
影响因子：
2.8
通讯作者：
Knudsen, S
中科院分区：
文献类型：
--
作者：
Jarmer, H;Larsen, TS;Knudsen, S
在实验验证的Sigma(A)识别位点的基础上，构建了枯草杆菌Sigma(A)RNA聚合酶辅因子识别位点的隐马尔可夫模型，该模型包含公共基序或扩展的-10基序。这项工作表明，在这两种类型的启动子中，转录起始点存在的信息比之前认为的更多。当在整个枯草杆菌基因组上进行测试时，该模型预测大约一半的sigma(A)识别位点是扩展类型。一些反应调节因子天冬氨酸磷酸酶是含有扩展位点的启动子的预测之一。定点突变证实RAPA和RAPB的表达依赖于延伸的-10区域。
A hidden Markov model of sigma (A) RNA polymerase cofactor recognition sites in Bacillus subtilis, containing either the common or the extended -10 motifs, has been constructed based on experimentally verified sigma (A) recognition sites. This work suggests that more information exists at the initiation site of transcription in both types of promoters than previously thought. When tested on the entire B. subtilis genome, the model predicts that approximately half of the sigma (A) recognition sites are of the extended type. Some of the response-regulator aspartate phosphatases were among the predictions of promoters containing extended sites. The expression of rapA and rapB was confirmed by site-directed mutagenesis to depend on the extended -10 region.