The effects of blocking the actions of estrogen and progesterone on the rates of proliferation and apoptosis of cervical epithelial and stromal cells during the second half of pregnancy in rats.
The effects of blocking the actions of estrogen and progesterone on the rates of proliferation and apoptosis of cervical epithelial and stromal cells during the second half of pregnancy in rats.
复制标题
阻断雌激素和孕激素的作用对大鼠妊娠后半期宫颈上皮和基质细胞增殖和凋亡率的影响。
DOI：
10.1095/biolreprod.105.043984
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Sherwood,ODavid
中科院分区：
文献类型：
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作者：
Lee,Hyung-Yul;Sherwood,ODavid
在大鼠妊娠23天的后半段，当子宫颈急剧生长时，血清中卵巢激素松弛素、雌激素和孕激素水平升高。最近，我们证明松弛素有助于宫颈生长，促进细胞增殖和抑制细胞凋亡的宫颈细胞在妊娠后期。本研究的目的是确定雌激素和孕激素对子宫颈细胞的增殖和凋亡率的影响，在3天的时间间隔在大鼠妊娠的后半段。雌激素和孕激素的作用被s.c.阻断。分别注射雌激素拮抗剂ICI 182，780和孕酮拮抗剂RU 486。为了评价细胞增殖，将5′-溴-2 ′-脱氧尿苷皮下注射。收集宫颈前8 h。末端脱氧核苷酸转移酶介导的脱氧尿苷5′-三磷酸缺口末端标记法检测凋亡细胞。通过免疫组织化学鉴定了凋亡细胞，并通过形态学分析确定了这些过程发生的速率。阻断雌激素和孕激素的作用可降低晚期妊娠子宫颈上皮细胞和间质细胞的增殖率，增加其凋亡率。而阻断孕激素的作用则对妊娠中期宫颈上皮细胞和间质细胞的凋亡产生相反的影响。总之，本研究提供的证据表明，雌激素和孕激素，松弛素，有助于增加宫颈细胞含量在妊娠后期通过促进宫颈细胞增殖和抑制凋亡。
Serum levels of the ovarian hormones relaxin, estrogen, and progesterone are elevated during the second half of 23-day rat pregnancy when dramatic growth of the cervix occurs. Recently, we demonstrated that relaxin contributes to cervical growth by both promoting cell proliferation and inhibiting apoptosis of cervical cells during late pregnancy. The objective of this study was to determine the influence of estrogen and progesterone on the rates of proliferation and apoptosis of cervical cells at 3-day intervals during the second half of rat pregnancy. The actions of estrogen and progesterone were blocked with s.c. injections of estrogen antagonist ICI 182,780 and progesterone antagonist RU486, respectively. To evaluate cell proliferation, 5′-bromo-2′-deoxyuridine was injected s.c. 8 h before cervixes were collected. Terminal deoxynucleotidyl transferase-mediated deoxyuridine 5′-triphosphate nick end-labeling was used to detect apoptotic cells. Proliferating and apoptotic cells were identified by immunohistochemistry, and the rates at which these processes occurred were determined by morphometric analysis. Blocking the actions of estrogen and progesterone decreased the rates of proliferation and increased the rates of apoptosis of both cervical epithelial and stromal cells during late pregnancy. However, blocking the actions of progesterone had the opposite effects on apoptosis of both cervical epithelial and stromal cells during the middle of pregnancy. In conclusion, this study provides evidence that estrogen and progesterone, like relaxin, contribute to the increase in the cervical cell content during late pregnancy by promoting proliferation and inhibiting apoptosis of cervical cells.