Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
复制标题
暴露于二氯二苯基三氯乙烷 (DDT)、三丁基锡 (TBT) 和 2,3,7,8-四氯二苯并-对二恶英 (TCDD) 的嗜热四膜虫的毒理学研究
DOI：
10.1007/s11427-011-4194-6
复制
发表时间：
2011-07-01
影响因子：
9.1
通讯作者：
Miao Wei
中科院分区：
文献类型：
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作者：
Chang Yue;Feng LiFang;Miao Wei
二氯二苯基三氯乙烷 (DDT)、三丁基锡 (TBT) 和 2,3,7,8-四氯二苯并-对二恶英 (TCDD) 在环境中持久存在，并对人类和水生生物造成持续的毒性影响。嗜热四膜虫有潜力用作毒物研究的模型。在这项研究中，这种生物体被用来分析由暴露于 DDT、TBT 和 TCDD 的细胞生成的全基因组微阵列。为了实现这一目标，我们鉴定了用每种毒物处理时差异表达的基因，然后使用 GO 富集分析对它们的功能进行分类。结果表明，嗜热木霉的反应与多细胞生物相似。此外，应用上下文相关性方法（CLR）来构建 TCDD 相关网络。获得的T形网络可以在功能上分为两个子网络。两个子网络的一般功能均与TCDD的表观遗传机制相关。基于网络分析，推断出 TCDD 对嗜热木霉的影响模型。因此，四膜虫有潜力成为基因组水平毒性机制研究的良好单细胞真核模型。
Dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic life. Tetrahymena thermophila has the potential for use as a model for research regarding toxicants. In this study, this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT, TBT and TCDD. To accomplish this, genes differentially expressed when treated with each toxicant were identified, after which their functions were categorized using GO enrichment analysis. The results suggested that the responses of T. thermophila were similar to those of multicellular organisms. Additionally, the context likelihood of relatedness method (CLR) was applied to construct a TCDD-relevant network. The T-shaped network obtained could be functionally divided into two subnetworks. The general functions of both subnetworks were related to the epigenetic mechanism of TCDD. Based on analysis of the networks, a model of the TCDD effect on T. thermophila was inferred. Thus, Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.