Population structure of wild wheat D-genome progenitor Aegilops tauschii Coss.: implications for intraspecific lineage diversification and evolution of common wheat
Population structure of wild wheat D-genome progenitor Aegilops tauschii Coss.: implications for intraspecific lineage diversification and evolution of common wheat
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DOI：
10.1111/j.1365-294x.2010.04537.x
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发表时间：
2010-03-10
影响因子：
4.9
通讯作者：
Takumi, Shigeo
中科院分区：
文献类型：
--
作者：
Mizuno, Nobuyuki;Yamasaki, Masanori;Takumi, Shigeo
节节山羊草 Coss.是六倍体小麦的D基因组祖先。节节山羊草是一种野生二倍体物种，在欧亚大陆中部有广泛的自然物种范围，从土耳其传播到中国西部。使用总共 122 个 Ae 种质进行扩增片段长度多态性 (AFLP) 分析。对节节小麦进行研究是为了阐明这种广泛分布的野生小麦物种的种群结构。系统发育和主成分分析揭示了伊蚊的两个主要谱系。节节。基于AFLP数据的贝叶斯种群结构分析表明，谱系一（L1）和谱系二（L2）分别显着分为6个和3个亚谱系。六个 L1 亚系中只有四个从外高加索和伊朗北部地区的西部栖息地分化到巴基斯坦和阿富汗等东部栖息地。包括L2在内的其他亚系在西部地区也有有限的分布。 strangulata 亚种似乎在 L2 的一个亚系中分化。先前在伊蚊中发现的三个主要单倍群（HG7、HG9 和 HG16）。基于叶绿体变异的节节藻群体，HG7种质广泛分布于L1和L2，HG9种质仅限于L2，HG16种质属于L1，表明HG9和HG16是由核基因组前两个谱系分化后的HG7形成的。这些结果对 Ae 的种群结构有影响。 tauschii 与 Ae 之间的谱系关系。节节草种质应提供有关遗传资源和自然遗传多样性保护的重要农业和进化知识。
Aegilops tauschii Coss. is the D-genome progenitor of hexaploid wheat. Aegilops tauschii, a wild diploid species, has a wide natural species range in central Eurasia, spreading from Turkey to western China. Amplified fragment length polymorphism (AFLP) analysis using a total of 122 accessions of Ae. tauschii was conducted to clarify the population structure of this widespread wild wheat species. Phylogenetic and principal component analyses revealed two major lineages in Ae. tauschii. Bayesian population structure analyses based on the AFLP data showed that lineages one (L1) and two (L2) were respectively significantly divided into six and three sublineages. Only four out of the six L1 sublineages were diverged from those of western habitats in the Transcaucasia and northern Iran region to eastern habitats such as Pakistan and Afghanistan. Other sublineages including L2 were distributed to a limited extent in the western region. Subspecies strangulata seemed to be differentiated in one sublineage of L2. Among three major haplogroups (HG7, HG9 and HG16) previously identified in the Ae. tauschii population based on chloroplast variation, HG7 accessions were widely distributed to both L1 and L2, HG9 accessions were restricted to L2, and HG16 accessions belonged to L1, suggesting that HG9 and HG16 were formed from HG7 after divergence of the first two lineages of the nuclear genome. These results on the population structure of Ae. tauschii and the genealogical relationship among Ae. tauschii accessions should provide important agricultural and evolutionary knowledge on genetic resources and conservation of natural genetic diversity.