Selenium blocks porcine circovirus type 2 replication promotion induced by oxidative stress by improving GPx1 expression
Selenium blocks porcine circovirus type 2 replication promotion induced by oxidative stress by improving GPx1 expression
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DOI：
10.1016/j.freeradbiomed.2012.04.035
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发表时间：
2012-08-01
影响因子：
7.4
通讯作者：
Huang, Kehe
中科院分区：
文献类型：
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作者：
Chen, Xingxiang;Ren, Fei;Huang, Kehe
猪圆环病毒2型（Porcine circovirus type 2，PCV 2）是引起断奶后多系统衰竭综合征（postweaning multisystemic wasting syndrome，PMWS）的重要病原，但并非所有感染PCV 2的猪都会发生PMWS。本研究的目的是在PCV 2感染的PK-15细胞培养模型中探索PCV 2感染、氧化应激和硒之间的关系。结果表明，氧化应激诱导的H2 O2处理增加PCV 2的复制作为衡量PCV 2的DNA拷贝数和感染的细胞数。此外，PCV 2复制抑制硒蛋氨酸（SeMet）在高浓度（6 μ M）和增加PCV 2复制的氧化应激被阻断SeMet在生理浓度（2或4 μ M）。PCV 2感染引起谷胱甘肽过氧化物酶1（GPx 1）活性降低，但GPx 1 mRNA水平升高，提示GPx 1可能是PCV 2感染过程中的一种重要防御机制。SeMet没有显著阻断GPx 1敲低细胞中PCV 2复制的促进作用。这一观察结果与SeMet对GPx 1基因敲减细胞中GPx 1 mRNA和活性的影响相关，表明GPx 1在阻断PCV 2复制的促进中起关键作用。我们的结论是，在不同的猪场观察到的PMWS的发病率和严重程度的差异可能与氧化应激的变化，硒有一个潜在的作用，在控制PCV 2感染。(c)2012 Elsevier Inc. All rights reserved.
Porcine circovirus type 2 (PCV2) is recognized as a key infectious agent in postweaning multisystemic wasting syndrome (PMWS), but not all pigs infected with PCV2 will develop PMWS. The aim of this work was to explore the relationships among PCV2 infection, oxidative stress, and selenium in a PK-15 cell culture model of PCV2 infection. The results showed that oxidative stress induced by H2O2 treatment increased PCV2 replication as measured by PCV2 DNA copies and the number of infected cells. Furthermore, PCV2 replication was inhibited by selenomethionine (SeMet) at a high concentration (6 mu M) and the increase in PCV2 replication by oxidative stress was blocked by SeMet at physiological concentrations (2 or 4 mu M). PCV2 infection caused a decrease in glutathione peroxidase 1 (GPx1) activity but an increase in GPx1 mRNA levels, suggesting that GPx1 may represent an important defense mechanism during PCV2 infection. SeMet did not significantly block the promotion of PCV2 replication in GPx1-knockdown cells. This observation correlates with the observed influence of SeMet on GPx1 mRNA and activity in GPx1-knockdown cells, indicating that GPx1 plays a key role in blocking the promotion of PCV2 replication. We conclude that differences in morbidity and severity of PMWS observed on different pig farms may be related to variations in oxidative stress and that selenium has a potential role in the control of PCV2 infection. (c) 2012 Elsevier Inc. All rights reserved.