Transient folate deprivation facilitates the generation of mouse‐induced pluripotent stem cells
Transient folate deprivation facilitates the generation of mouse‐induced pluripotent stem cells
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DOI：
10.1002/cbin.10233
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发表时间：
2014-05
影响因子：
3.9
通讯作者：
Qiuyue Yan;Jie Xu;Wen-tao Hu;Zhen Li;Jun Wu;Su-ming Zhang
中科院分区：
文献类型：
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作者：
Qiuyue Yan;Jie Xu;Wen-tao Hu;Zhen Li;Jun Wu;Su-ming Zhang
体细胞可以通过特定转录因子的异位表达重编程为诱导多能干细胞。然而，低效率和所需的时间量限制了iPS细胞的潜在应用。我们报道了一种通过叶酸剥夺从MEF（小鼠胚胎成纤维细胞）产生iPS细胞的有效方法，该方法用于改变MEF的甲基化。没有叶酸3天，诱导效率提高了5倍。核型分析表明，短暂的叶酸剥夺处理不会对iPS细胞的特性产生负面影响;表征的iPS细胞显示正常的核型。因此，我们发现了一种新的方法，可以提高诱导效率，但不会增加染色体突变的机会。
Somatic cells can be reprogrammed into iPS (induced pluripotent stem) cells through the ectopic expression of defined transcription factors. However, the inefficiency and amount of time needed limited the potential application of iPS cells. We report an efficient method to generate iPS cells from MEF (mouse embryonic fibroblasts) through folate‐depriviatoin, which was used to change the methylation of MEF. Without folate for 3 days, the induction efficiency is enhanced fivefold. Karyotype analysis showed that transient folate‐depriving treatment did not negatively affect properties of iPS cells; characterised iPS cells show normal karyotypes. Thus, a new method has been found that can improve the induction efficiency, but not increase the chance of chromosomal mutation.