Permanent culture and parasitic impact of the microalga Coccomyxa parasitica, isolated from horse mussel Modiolus kurilensis.
Permanent culture and parasitic impact of the microalga Coccomyxa parasitica, isolated from horse mussel Modiolus kurilensis.
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从马贻贝 Modiolus kurilensis 中分离出的微藻 Coccomyxa parasitica 的永久培养和寄生影响。
DOI：
10.1016/j.jip.2016.07.012
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发表时间：
2016
影响因子：
3.4
通讯作者：
Vadim V Kumeiko
中科院分区：
文献类型：
--
作者：
Y. Sokolnikova;T. Magarlamov;A. Stenkova;Vadim V Kumeiko
在日本海彼得大湾马贻贝的自然种群中发现了贝壳变形和微藻入侵的动物。血淋巴最初充斥着藻类，然后是直肠、虹吸管、外套膜和位于身体后部的性腺。地衣主要暴露在光线下，是藻类的主要储藏库。对藻细胞的显微分析表明，藻细胞中没有鞭毛和类果核，只有一个叶绿体，通过自孢子繁殖，四分孢子上有分生孢子。这些结果，再加上对18S rDNA小亚基(SSU)1728个碱基的几乎完整的序列分析，证实了这些细胞是寄生球孢霉。一种新开发的从软体动物组织中分离微藻的方法促进了FC的连续纯培养--可能是无菌培养。寄生虫，从而提供对每个生命阶段的时间进程的描述。组织学分析表明，被微藻感染的外套膜、性腺、肾脏和消化腺组织以及鳃组织中有大量的血细胞渗透，其中没有确定入侵者的身份。在这些被感染的器官中发现了藻类包裹，主要是纤维化和无定形坏死的灶性区域。性腺滤泡和消化腺管之间的间隙明显变宽，因为这些区域充满了大量的藻类和吞噬的血细胞，显示出基底膜增厚的腺泡。外套膜和肾脏在上皮组织、结缔组织和肌肉组织中表现出明显的不同细胞的形态变化，导致寄生器官功能障碍。因此，无论寄主是什么，在培养物中繁殖的寄生虫是一种兼性寄生虫，可引起组织形态异常和寄生器官功能障碍等重大病理改变。
Animals with deformed shells and microalgal invasion have been identified in the natural population of the horse mussel speciesModiolus kurilensisof Peter the Great Bay in the Sea of Japan. The haemolymph is initially infested with algae, followed by the rectum, siphons, mantles and gonads located in the posterior body areas. Mantles, which are primarily exposed to light, are major depots for algae. The microscopic analysis of algal cells has revealed the absence of flagella and pyrenoids, the presence of single chloroplast, and reproduction by autosporulation, with dispores prevailing over tetraspores. These results, together with the nearly complete sequence analysis of small subunit (SSU) 18S rDNA (1728 bp), have confirmed that these cells areCoccomyxa parasitica. A newly developed method of isolating microalgae from mollusk tissues has facilitated the continuous pure - probably axenic - culture ofC. parasitica, thereby providing a description of the time course of each life stage. Histological analyses have revealed significant haemocyte infiltration into the mantles, gonads, kidneys and digestive gland tissues infested with microalgae and the gill tissues, in which the intruder was not identified. Algal encapsulation with major focal areas of fibrosis and amorphic necrosis has been revealed in these infested organs. The spaces between the gonad follicles and digestive gland tubules were significantly widened as these areas were filled with a mass of algae and phagocytic haemocytes, showing acini with a thickened basement membrane. The mantles and kidneys ofModiolusdisplayed significant morphological deviations of different cells in epithelial, connective and muscle tissues, resulting in the dysfunction of the infested organs. Therefore,C. parasitica, which reproduces in the culture, regardless of the host, is a facultative parasite, causing major pathological alterations, such as anomalous histomorphological patterns and infested organ dysfunctions.
影响因子：
10.7
作者：
Tamura, Koichiro;Dudley, Joel;Kumar, Sudhir
通讯作者：
Kumar, Sudhir