Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study
Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study
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DOI：
10.1034/j.1600-051x.2003.00001.x
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发表时间：
2003-04-01
影响因子：
6.7
通讯作者：
d'Hoedt, B
中科院分区：
文献类型：
--
作者：
Kreisler, M;Christoffers, AB;d'Hoedt, B
目的：本体外研究的目的是评估低强度激光照射对人牙周膜成纤维细胞（PDLF.Materials and Methods）增殖的潜在刺激作用：在标准条件下培养第三磨牙牙周膜成纤维细胞，并将其铺在96孔组织培养板上。用809 nm的二极管激光器以10 mW的功率输出在连续波（cw）模式下以1.96-7.84 Jcm（-2）的能量注量照射亚融合单层。可变辐照参数为暴露时间（75-300 s/孔）和辐照次数（1-3）。激光处理后，培养物孵育24小时。通过添加到细胞培养物中的还原-氧化（REDOX）指示剂（Alcohol Blue（R）Assay）的荧光活性来测定激光处理和对照培养物的增殖速率。增殖，相对荧光单位（RFU）表示，测定照射后24，48和72 h。结果：照射后的细胞表现出相当高的增殖活性比对照组。照射后72 h差异显著（Mann-Whitney U检验，p < 0.05）。结论：软激光应用的细胞效应清晰可辨。临床研究需要评估低强度激光治疗的应用是否可能有利于牙周再生治疗。
Aim: The aim of this in vitro study was to evaluate a potential stimulatory effect of low-level laser irradiation on the proliferation of human periodontal ligament fibroblasts (PDLF).Materials and Methods: PDLF obtained from third molar periodontal ligaments were cultured under standard conditions and spread on 96-well tissue culture plates. Subconfluent monolayers were irradiated with an 809-nm diode laser operated at a power output of 10 mW in the continuos wave (cw) mode at energy fluences of 1.96-7.84 Jcm(-2). The variable irradiation parameters were the time of exposure (75-300 s per well) and the number of irradiations (1-3). After laser treatment, the cultures were incubated for 24 h. The proliferation rate of the lased and control cultures was determined by means of fluorescence activity of a reduction-oxidation (REDOX) indicator (Alamar Blue(R) Assay) added to the cell culture. Proliferation, expressed in relative fluorescence units (RFU), was determined 24, 48 and 72 h after irradiation.Results: The irradiated cells revealed a considerably higher proliferation activity than the controls. The differences were significant up to 72 h after irradiation (Mann-Whitney U-test, p < 0.05).Conclusion: A cellular effect of the soft laser application is clearly discernible. Clinical studies are needed to evaluate whether the application of low-level laser therapy might be beneficial in regenerative periodontal therapy.