Pseudomonas aeruginosa pvdQ Gene Prevents Caco-2 Cells from Obstruction of Quorum-Sensing Signal
Pseudomonas aeruginosa pvdQ Gene Prevents Caco-2 Cells from Obstruction of Quorum-Sensing Signal
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铜绿假单胞菌 pvdQ 基因防止 Caco-2 细胞群体感应信号受阻
DOI：
10.1007/s00284-010-9668-4
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ye L, Li G, Li H, Wang L, Mao Y, Xie X, Xia C, Che
中科院分区：
文献类型：
--
作者：
Ye L, Li G, Li H, Wang L, Mao Y, Xie X, Xia C, Che
群体感应（Quorum sensing，QS）系统在难治性铜绿假单胞菌感染的致病过程中起着重要作用，这为目前尚无有效治疗方法的铜绿假单胞菌感染提供了新的治疗策略。最新研究表明，铜绿假单胞菌pvdQ基因可通过编码酰化酶，水解QS系统的关键信号分子N-（3-氧代十二烷酰基）-高丝氨酸内酯（3 O-oxo-C12-HSL），从而减弱细菌的致病性。本研究试图通过pvdQ基因转染人肠上皮细胞Caco-2来对抗铜绿假单胞菌的致病性。我们发现转染pvdQ基因的细胞上清中3 O-oxo-C12-HSL减少。流式细胞仪检测结果显示，转染pvdQ基因的Caco-2细胞对3 O-oxo-C12-HSL诱导的细胞凋亡率有明显的抑制作用。而对照组在未加pvdQ基因保护的情况下，Caco-2细胞经3 O-oxo-C12-HSL刺激后凋亡率增加。结论：通过pvdQ基因的导入，我们成功地保护了哺乳动物细胞Caco-2免受QS信号分子3 O-oxo-C12-HSL的损伤，这可能为铜绿假单胞菌感染的治疗提供了一种新的策略。
Quorum sensing (QS) system plays an important role in bacterial pathopoiesis of incurable Pseudomonas aeruginosa infection, which strongly warrants new strategies for absence of curative treatment to date. Latest investigations show that pvdQ gene of P. aeruginosa can attenuate the pathopoiesis of the bacteria by encoding acylase enzyme and hydrolyze N-(3-oxododecanoyl)-Homoserine Lactone (3O-oxo-C12-HSL), the key signal molecule of QS system. This study tries to resist the pathogenicity of P. aeruginosa by transfecting human intestinal epithelial Caco-2 cells with pvdQ gene. We found that 3O-oxo-C12-HSL was decreased in the supernatant of cells transfected with pvdQ gene. Moreover, the result of flow cytometry showed that the 3O-oxo-C12-HSL evoked apoptosis rate of Caco-2 cells was inhibited when the cells were transfected with pvdQ gene. In contrast, the control result displayed increased Caco-2 cells’ apoptosis rate after stimulation of 3O-oxo-C12-HSL without protection of pvdQ gene. In conclusion, we successfully protect mammalian cells Caco-2 from injure of QS signal molecule 3O-oxo-C12-HSL through imputing pvdQ gene, which may suggest a new therapeutic strategy for P. aeruginosa infection.
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DOI：
10.1152/ajpgi.00241.2004
发表时间：
2005-05-01
影响因子：
4.5
作者：
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影响因子：
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作者：
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