Nerve growth factor induced hyperalgesia in the rat hind paw is dependent on circulating neutrophils
Nerve growth factor induced hyperalgesia in the rat hind paw is dependent on circulating neutrophils
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DOI：
10.1016/s0304-3959(98)00114-6
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发表时间：
1998-09-01
期刊：
影响因子：
7.4
通讯作者：
Brain, SD
中科院分区：
文献类型：
--
作者：
Bennett, G;al-Rashed, S;Brain, SD
研究了神经生长因子（NGF）诱导大鼠热痛觉过敏和中性粒细胞积累的机制。用脊髓过氧化物酶测定法测量大鼠后爪的热痛觉阈值，并测量后爪和背部皮肤样本的中性粒细胞积累。NGF （23-80 pmol足底注射）在注射后5 h诱导了显著的（P < 0.05, n = 6-16）热痛觉过敏，NGF （40 pmol）组出现了显著的中性粒细胞积累（P < 0.05, n = 6）。在背部皮肤，可以评估多个样本，皮内（i.d）。NGF诱导中性粒细胞积累的效力比白细胞介素-1 β低10-30倍。5-脂氧合酶抑制剂ZM230487 （10 nmol与NGF共注射）显著减轻NGF引起的中性粒细胞积累和痛觉过敏；不像组胺和5-羟色胺拮抗剂（甲皮拉米和甲塞吉特）在泰晤士报测量中没有效果。此外，消耗循环中性粒细胞（使用兔抗大鼠中性粒细胞抗体）可消除NGF诱导的痛觉过敏。这些结果表明，中性粒细胞在ngf诱导的痛觉过敏中起着至关重要的作用，中性粒细胞在5-脂氧合酶产物的反应中积累。(C) 1998国际疼痛研究协会。Elsevier Science B.V.出版
The mechanisms by which nerve growth factor (NGF) induces thermal hyperalgesia and neutrophil accumulation have been investigated in the rat. Thermal nociceptive thresholds in rat hind paw were measured as the time taken for paw withdrawal from a heat source and neutrophil accumulation was measured in hind paw and dorsal skin samples using a myeloperoxidase assay. NGF (23-80 pmol intraplantar (i.pl.) injection) induced a significant (P < 0.05, n = 6-16) thermal hyperalgesia at 5 h after injection and significant neutrophil accumulation (P < 0.05, n = 6) was observed with NGF (40 pmol). In dorsal skin, where multiple samples can be assessed, intradermal (i.d.) NGF was 10-30 times less potent than interleukin-1 beta in inducing neutrophil accumulation. The 5-lipoxygenase inhibitor ZM230487 (10 nmol co-injected with NGF) significantly attenuated neutrophil accumulation and hyperalgesia induced by NGF; unlike the histamine and 5-hydroxytryptamine antagonists (mepyramine and methysergide) which were without effect at the times measured. Furthermore, depletion of circulating neutrophils (using a rabbit anti-rat neutrophil antibody) abolished NGF induced hyperalgesia. These results indicate that neutrophils, which accumulate in response to a 5-lipoxygenase product, play a crucial role in NGF-induced hyperalgesia. (C) 1998 International Association for the Study of Pain. Published by Elsevier Science B.V.